CAREER: Coordinating Primary and Secondary Metabolic Activities for Enhancing Terpenoid Indole Alkaloid Production from Catharanthus roseus Cultures

本研究的目的是开发一种技术，以显著提高玫瑰花细胞培养中萜类吲哚生物碱（TIA）的产量。提出的假设是，为了最大限度地产生次生代谢物，如TIAs，前体和能量资源在各个水平上的相对流动必须平衡。拟议的具体目标是实现这一平衡，以增加TIA生产：(1)增加初级和次级代谢的通量；(2)最大化TIA产生的代谢通量，同时保持适当的细胞机制代谢通量，以支持必需的细胞活动；(3)调节糖酵解、柠檬酸和戊糖磷酸途径之间的通量，以最大限度地产生次级代谢物；(4)表征各种途径和活动的最佳平衡。本研究结果可提高重要抗肿瘤和降压化合物的产量。

0134511LeeThe objective of the proposed research is to develop techniques to significantly increase terpenoid indole alkaloid (TIA) production from cell cultures of C. roseus. The proposed hypothesis is that to maximize the production of secondary metabolites such as TIAs, the relative flows of precursors and energy resources at various levels must be balanced. The proposed specific aims for achieving this balance for increasing TIA production are to: (1) increase flux to both primary and secondary metabolism, (2) maximize the metabolic flux to TIA production while maintaining an appropriate metabolic flux to cellular machinery for supporting essential cellular activities, (3) tune the flux between the glycolysis, citric acid, and pentose phosphate pathways for maximum secondary metabolite production, and (4) characterize the optimum balance the various pathways and activities. This research could produce results to increase the yield of important antitumor and antihypertensive compounds.