POWRE: Directing Indole Alkaloid Synthesis from Catharanthus roseus Cultures

本课题的目的是设计一种从固定化长春花中生产和回收长春花碱的工艺。roseus也叫。在此过程中，培养基将在生物反应器和吸附柱之间循环。优化产物去除率以提高产量，最大限度地从细胞中回收产物，并维持培养物的长期生产。该项目的具体目标是确定影响ajmalicine生产和回收的步骤，确定其相关动力学，然后将这些速率纳入模型，然后可以应用于优化树脂添加策略。

The goal of this project is to design a process for the production and recovery of ajmalicine from immobilized Catharanthus. roseus. In this process, the medium would be cycled between the bioreactor and the adsorbent column. The rate of product removal would be optimized to improve production, maximize recovery of product from the cell, and sustain long-term production of the cultures. Specific objectives of this project are to identify the steps which affect the production and recovery of ajmalicine, determine their associated kinetics, and then incorporate these rates into a model which can then be applied to optimize the strategy of resin addition.