BAC Libraries from Diverse Crustacean Taxa

生物学的基本目标之一是了解基因组结构和功能如何随环境变化而进化，以及遗传限制如何限制物种的自然分布、丰度和适应性进化。为了实现这些目标，托马斯博士提议为不同的甲壳类动物开发细菌人工染色体(BAC)文库。这些图书馆专门用于支持比较发育研究和关于生物体如何应对环境变化的调查。甲壳动物是比较发育研究的理想动物，因为它们具有非凡的形态多样性，并且与主要的发育模式生物--黑腹果蝇关系密切。甲壳动物类群也是许多生态学研究的重点，目的是了解生物与其环境之间的基本相互作用。拟议的BAC文库资源及其分布将为支持发展进化和环境基因组学提供关键工具。托马斯博士特别建议为代表三个甲壳纲的三个分类群开发BAC文库。以水蚤(Daphnia Pulex)为代表的支脚类动物。长足纲由尺足类Tigriopus calforNicus代表，软体纲由端足类Parhyale hawasis代表。Daphnia和Parhyale已经是许多实验室进行比较发育研究的对象。这项工作将与一组主要用户合作进行，他们积极参与这项提议，就适当的分类群进行咨询，提供用于DNA提取的标本，并帮助使这些资源可用于其特定于分类群或学科的社区。这些合作者代表了生物学研究的不同领域，并代表了潜在用户群体的广泛范围。每个文库的生成和分配将在新汉普郡大学哈伯德基因组研究中心(HCGs)进行，由PI(该中心的联席主任)指导。HCGs的主要重点是比较基因组学和环境基因组学，并将结合这项提议提供关于基因组技术的密集夏季讲习班，以支持学生和博士后使用BAC图书馆。来自每个分类群的BAC图书馆资源将以两种方式提供给所有学术和非学术用户：1)作为图书馆的完整排列副本；2)在尼龙过滤器上以高密度排列。BAC资源和相关信息将通过WWW访问。授权机构将在授权期结束后至少五年内维持和分配这些资源。

.0208364ThomasOne of the fundamental goals of biology is to understand how genome structure and function evolves in response to environmental change and how genetic constraints limit the natural distribution, abundance, and adaptive evolution of species. Toward these goals, Dr. Thomas is proposing to develop Bacterial Artificial Chromosome (BAC) libraries for diverse crustacean taxa. These libraries are specifically intended to support both comparative developmental studies and investigations into how organisms respond to environmental change. The Crustacea are ideal for comparative developmental studies because of their extraordinary morphological diversity, and close relationship to the premier developmental model organism, Drosophila melanogaster. Crustacean taxa are also the focus of many ecological studies aimed at understanding the fundamental interactions between organisms and their environment. The proposed BAC library resources and their distribution will provide critical tools in support of developmental evolution and environmental genomics. Dr. Thomas is specifically proposing to develop BAC libraries for three taxa representing three crustacean classes. The Branchiopods are represented by the water flea, Daphnia pulex. The Maxillapoda are represented by a copepod Tigriopus californicus, and the class Malacostraca is represented by an amphipod, Parhyale hawaiensis. Daphnia and Parhyale are already the subjects of comparative developmental studies in numerous laboratories. This work will take place in collaboration with a set of primary users who are actively participating in this proposal by consulting on the appropriate taxa, supplying specimens for DNA extraction and helping to make these resources available and useful to their taxon- or discipline-specific communities. These collaborators represent disparate fields of biological research and represent a broad spectrum of the potential user community. The generation and distribution of each library will take place at the Hubbard Center for Genome Studies (HCGS) at the University of New Hampshire under the direction of the PI (co-director of the center). The HCGS has a primary focus of comparative and environmental genomics, and in conjunction with this proposal will be offering intensive summer workshops in genomic technologies to support the use of BAC libraries by students and postdocs. BAC library resources from each taxon will be made available to all academic and non-academic users in two ways: 1) as complete arrayed copies of the library; and 2) arrayed at high density on nylon filters. The BAC resources and associated information will be accessible via the WWW. The HCGS will maintain and distribute these resources for a minimum of five years after the end of the granting period.