NER: Oligonucleotide-Directed Alignment of Cytoskeletal Filaments for Nanoscale Assembly

NER：用于纳米级组装的细胞骨架细丝的寡核苷酸定向排列目前，人们对纳米科学和纳米技术在亚微米尺度上操纵材料非常感兴趣。然而，在细胞中，这是一项常规任务--细胞内货物的运输是由马达蛋白沿着称为微管的细胞骨架细丝移动进行的。实现定向运动是因为微管具有结构极性，马达沿着它们单向运动，不同的马达朝着不同的方向运动。我们寻求：(I)使用纯化的运动蛋白马达蛋白和微管在体外重建细胞内运输系统，以及(Ii)使用它将特定的货物(生物或合成纳米级物体)运输到二维底物上的特定位置。为了铺设具有所需取向的微管轨迹，我们将利用DNA杂交作为“分子胶”的特异性和可逆性。单链DNA寡核苷酸将被连接到玻璃表面的特定位置，互补的寡核苷酸将被共价连接到微管上。通过制作末端用不同DNA序列功能化的微管，并对其序列互补进行空间构图，将研究各种微管几何形状。这些轨道将被用来指导装载货物的动蛋白马达的运动，作为建立生物分子分离或定向组装应用的系统的一步。

NER: Oligonucleotide-directed alignment of cytoskeletal filaments for nanoscale assemblyThere is currently great interest in nanoscience and nanotechnology for manipulating material at submicron scales. In cells, however, this is a routine task -- the transport of intracellular cargo is carried out by motor proteins that move along cytoskeletal filaments called microtubules. Directional movement is achieved because microtubules have a structural polarity and motors move unidirectionally along them, with different motors moving in different directions. We seek to: (i) reconstruct the intracellular transport system in vitro using purified kinesin motor proteins and microtubules, and (ii) use it to transport defined cargo (biological or synthetic nanoscale objects) to specific sites on a two-dimensional substrate. To lay down microtubule tracks with desired orientations, we will take advantage of the specificity and reversibility of DNA hybridization as a "molecular glue". Single-stranded DNA oligonucleotides will be attached to defined sites on glass surfaces and complementary oligonucleotides will be covalently attached to microtubules. By making microtubules whose ends are functionalized with different DNA sequences and spatially patterning their sequence complements, a variety of microtubule geometries will be investigated. These tracks will be used to direct the motion of cargo-laden kinesin motors as a step towards building systems for biomolecular separations or directed assembly applications.