NER: Oligonucleotide-Directed Alignment of Cytoskeletal Filaments for Nanoscale Assembly

NER：用于纳米级组装的细胞骨架丝的寡核苷酸定向排列

• 批准号: 0209687
• 负责人: William Hancock
• 金额: $ 9.5万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0209687&HistoricalAwards=false
• 关键词: NER; Oligonucleotide; Directed; Alignment; Cytoskeletal

NER: Oligonucleotide-directed alignment of cytoskeletal filaments for nanoscale assemblyThere is currently great interest in nanoscience and nanotechnology for manipulating material at submicron scales. In cells, however, this is a routine task -- the transport of intracellular cargo is carried out by motor proteins that move along cytoskeletal filaments called microtubules. Directional movement is achieved because microtubules have a structural polarity and motors move unidirectionally along them, with different motors moving in different directions. We seek to: (i) reconstruct the intracellular transport system in vitro using purified kinesin motor proteins and microtubules, and (ii) use it to transport defined cargo (biological or synthetic nanoscale objects) to specific sites on a two-dimensional substrate. To lay down microtubule tracks with desired orientations, we will take advantage of the specificity and reversibility of DNA hybridization as a "molecular glue". Single-stranded DNA oligonucleotides will be attached to defined sites on glass surfaces and complementary oligonucleotides will be covalently attached to microtubules. By making microtubules whose ends are functionalized with different DNA sequences and spatially patterning their sequence complements, a variety of microtubule geometries will be investigated. These tracks will be used to direct the motion of cargo-laden kinesin motors as a step towards building systems for biomolecular separations or directed assembly applications.

NER：利用寡核苷酸定向排列细胞骨架丝进行纳米级组装目前，人们对在亚微米尺度上操纵材料的纳米科学和纳米技术产生了极大的兴趣。 然而，在细胞中，这是一项常规任务--细胞内货物的运输是由马达蛋白进行的，马达蛋白沿着沿着称为微管的细胞骨架细丝移动。 定向运动的实现是因为微管具有结构极性，并且马达沿它们沿着单向运动，不同的马达沿不同的方向运动。 我们力求：（i）使用纯化的驱动蛋白马达蛋白和微管在体外重建细胞内转运系统，和（ii）使用它将限定的货物（生物或合成纳米级物体）转运到二维基底上的特定位点。 利用DNA杂交的特异性和可逆性作为“分子胶”，在微管上形成所需方向的轨道。 单链DNA寡核苷酸将连接到玻璃表面上的限定位点，互补寡核苷酸将共价连接到微管。 通过制造末端用不同DNA序列官能化的微管，并在空间上图案化它们的序列互补物，将研究各种微管几何形状。 这些轨道将被用来指导载货物的驱动蛋白马达的运动，作为构建生物分子分离或定向组装应用系统的一个步骤。