Biophotonics: Molecular Motor Biophotonics

该提案的具体目标是：1）设计和构建连接驱动蛋白分子马达的微加工通道，用于运输和分选微管。2）将荧光标记的DNA寡核苷酸（分子信标）连接到微管作为诱饵，通过荧光显微镜观察核酸货物的结合和运输。3）制造单片封装的有机发光二极管（OLED），其耦合到微管微通道作为局部源以激发荧光标记。4）制造集成的光电探测器，用于局部检测荧光信号。光电探测器将与OLED组合使用，以检测结合到在通道中行进的微管的荧光DNA。5）组装由连接到运输通道的样品储存器、检测器和分叉组成的系统，以从空微管中分选负载货物，以及收集储存器，以存款所选择的感兴趣的分子。

0323024HancockThe specific aims of this proposal are to:1) Design and construct microfabricated channels to which kinesin molecular motors are attached for transporting and sorting microtubules.2) Attach fluorescently labeled DNA oligonucleotides (molecular beacons) to microtubules as bait, visualize binding and transport of the nucleic acid cargo by fluorescence microscopy.3) Fabricate monolithically encapsulated organic light emitting diodes (OLEDs) with coupling to the microtubule microchannels as local sources to excite the fluorescent labels.4) Fabricate integrated photodetectors for local detection of fluorescence signals. The photodetectors will be used in combination with the OLEDs to detect fluorescent DNA bound to microtubules traveling in the channels.5) Assemble a system consisting of a sample reservoir connected to a transport channel, a detector, and bifurcation to sort cargo-laden from empty microtubules, and a collection reservoir to deposit the selected molecules of interest.