SGER: Inactivation of Biological Warfare Agents by Physical Disinfectants

SGER：物理消毒剂灭活生物战剂

• 批准号: 0210350
• 负责人: Ernest Blatchley
• 金额: $ 9.54万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至 2003-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0210350&HistoricalAwards=false
• 关键词: SGER; Inactivation; Biological; Warfare; Agents

0210350BlatchleyThe objective of the research is to investigate the feasibility and limitations of Bacillus anthracis decontamination processes which utilize the physical disinfectants ultraviolet (UV) and gamma (g) irradiation. The specific tasks of this exploratory research include: (1) the development of a database of the behavior for B. anthracis spores in response to UV and g irradiation, noting the spore inactivation, repair, and mutation, (2) the development of systems to quantify dose delivery in decontamination systems based on physical disinfectants using Bacillus cereus as a surrogate for B. anthracis spores, and (3) examination of spore inactivation in continuous applications to surfaces and aqueous liquids over a range of operating conditions, together with dosimetric data. The use of B. cereus as a surrogate is logical and useful, since B. cereus is virtually indistinguishable from B. anthracis in terms of physiology and responses to disinfectants, but is essentially non-pathogenic to humans. As a check of the validity of the use of B. cereus, a limited number of experiments will be performed with the Sterne strain of B. anthracis (the vaccine strain of the organism) to verify the results of the research.

本研究的目的是探讨利用物理消毒剂紫外线(UV)和伽马(G)照射的炭疽芽孢杆菌去污工艺的可行性和局限性。这项探索性研究的具体任务包括：(1)开发炭疽芽胞对紫外线和g辐射的响应行为数据库，记录孢子灭活、修复和突变；(2)开发系统，以蜡状芽孢杆菌作为炭疽芽胞的替代品，量化基于物理消毒剂的去污系统中的剂量传递；以及(3)检查在一系列操作条件下连续应用于表面和水溶液中的孢子灭活情况，以及剂量测量数据。使用蜡样芽孢杆菌作为替代品是合乎逻辑和有用的，因为蜡样芽孢杆菌在生理和对消毒剂的反应方面与炭疽杆菌几乎没有区别，但基本上对人类没有致病性。为了检查使用蜡样芽孢杆菌的有效性，将对炭疽杆菌的斯特恩菌株(生物体的疫苗菌株)进行有限数量的实验，以验证研究结果。