SGER: Inactivation of Biological Warfare Agents by Physical Disinfectants

本研究的目的是探讨利用物理消毒剂紫外（UV）和γ (g)照射对炭疽芽孢杆菌进行去污的可行性和局限性。本探索性研究的具体任务包括：(1)建立一个数据库，记录炭疽芽孢杆菌孢子在紫外线和g辐射下的行为，注意到孢子的失活、修复和突变；(2)开发系统，以蜡样芽孢杆菌作为炭疽芽孢杆菌孢子的替代品，在基于物理消毒剂的去污系统中量化剂量传递；(3)在一系列操作条件下连续应用于表面和水性液体的孢子失活检查。以及剂量学数据。使用蜡样芽孢杆菌作为替代品是合乎逻辑且有用的，因为蜡样芽孢杆菌在生理学和对消毒剂的反应方面实际上与炭疽芽孢杆菌难以区分，但基本上对人类无致病性。为了检验蜡样芽孢杆菌使用的有效性，将使用炭疽芽孢杆菌的斯特恩菌株（该生物的疫苗菌株）进行有限数量的实验，以验证研究结果。

0210350BlatchleyThe objective of the research is to investigate the feasibility and limitations of Bacillus anthracis decontamination processes which utilize the physical disinfectants ultraviolet (UV) and gamma (g) irradiation. The specific tasks of this exploratory research include: (1) the development of a database of the behavior for B. anthracis spores in response to UV and g irradiation, noting the spore inactivation, repair, and mutation, (2) the development of systems to quantify dose delivery in decontamination systems based on physical disinfectants using Bacillus cereus as a surrogate for B. anthracis spores, and (3) examination of spore inactivation in continuous applications to surfaces and aqueous liquids over a range of operating conditions, together with dosimetric data. The use of B. cereus as a surrogate is logical and useful, since B. cereus is virtually indistinguishable from B. anthracis in terms of physiology and responses to disinfectants, but is essentially non-pathogenic to humans. As a check of the validity of the use of B. cereus, a limited number of experiments will be performed with the Sterne strain of B. anthracis (the vaccine strain of the organism) to verify the results of the research.