Rapid and Selective Protein Purification via pH-Tunable Water-in-Oil Microemulsions

通过 pH 可调的油包水微乳液快速、选择性地纯化蛋白质

• 批准号: 0211164
• 负责人: Douglas Hayes
• 金额: $ 33.11万
• 依托单位: University of Alabama in Huntsville
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211164&HistoricalAwards=false
• 关键词: Rapid; Selective; Protein; Purification; via

The goal of this project is to employ microemulsion-based protein extraction (MPE) systems for the rapid, selective, and scalable recovery and purification of proteins. The research plan consists of first investigating the physical chemistry and hydrolysis kinetics of the mixed surfactant systems, to determine difference between this mixed surfactant system with comparable ionic/nonionic mixed systems, and to identify operating conditions for MPE. In addition, the mixed surfactant system will be applied to the MPE of single- and multiple protein systems, the latter systems related to the fractionation of blood plasma proteins. The success of MPE will be evaluated based on productivity (i.e., speed and protein capacity), selectivity, applicability to a wide-range of operating conditions and protein purification challenges, and non-inactivation of protein products. Furthermore, pH-degradable ionic and bioaffinity water-in-oil microemulsions forming surfactants will be synthesized and employed.

该项目的目标是采用基于微乳液的蛋白质提取（MPE）系统，用于蛋白质的快速，选择性和可扩展的回收和纯化。 研究计划包括首先调查混合表面活性剂体系的物理化学和水解动力学，以确定该混合表面活性剂体系与可比的离子/非离子混合体系之间的差异，并确定MPE的操作条件。 此外，混合表面活性剂系统将被应用于单蛋白和多蛋白系统的MPE，后者系统涉及血浆蛋白的分级分离。 MPE的成功将根据生产率（即，速度和蛋白质容量）、选择性、对广泛的操作条件和蛋白质纯化挑战的适用性以及蛋白质产物的非失活。 此外，pH可降解的离子和生物亲和性的油包水型微乳液形成的表面活性剂将被合成和使用。