Rapid and Selective Protein Purification via pH-Tunable Water-in-Oil Microemulsions

该项目的目标是采用微乳化蛋白提取（MPE）系统快速，选择性和可扩展的蛋白质回收和纯化。研究计划包括首先研究混合表面活性剂体系的物理化学和水解动力学，确定这种混合表面活性剂体系与同类离子/非离子混合体系的区别，并确定MPE的操作条件。此外，混合表面活性剂体系将应用于单蛋白和多蛋白体系的MPE，后者与血浆蛋白的分离有关。MPE的成功将根据生产率（即速度和蛋白质容量）、选择性、对广泛操作条件和蛋白质纯化挑战的适用性以及蛋白质产物的非失活性来评估。此外，还将合成可降解的离子型和生物亲和的油包水微乳液形成表面活性剂。

The goal of this project is to employ microemulsion-based protein extraction (MPE) systems for the rapid, selective, and scalable recovery and purification of proteins. The research plan consists of first investigating the physical chemistry and hydrolysis kinetics of the mixed surfactant systems, to determine difference between this mixed surfactant system with comparable ionic/nonionic mixed systems, and to identify operating conditions for MPE. In addition, the mixed surfactant system will be applied to the MPE of single- and multiple protein systems, the latter systems related to the fractionation of blood plasma proteins. The success of MPE will be evaluated based on productivity (i.e., speed and protein capacity), selectivity, applicability to a wide-range of operating conditions and protein purification challenges, and non-inactivation of protein products. Furthermore, pH-degradable ionic and bioaffinity water-in-oil microemulsions forming surfactants will be synthesized and employed.