Nährstoffarme Emers-Fermentation terrestrischer Cyanobakterien zur Produktion biotechnischer Wertstoffe

陆地蓝藻的营养贫乏 Emers 发酵用于生产生物技术有价值的材料

• 批准号: 158354519
• 负责人: Dr. Michael Lakatos
• 金额: --
• 依托单位: Lehrgebiet Bioverfahrenstechnik (BioVT)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/158354519?language=en
• 关键词: hrstoffarme; Emers; Fermentation; terrestrischer; Cyanobakterien

Terrestrial cyanobacteria are characterized by a great variety of valuables, which differ fundamentally from aquatic organisms. At the moment, the exploitation of these valuables and the production of larger quantities of these metabolites are not realized because the cultivation technology is unavailable yet. Therefore, we developed a novel type of emerse photobioreactor, within the first period of the project, to cultivate this group of organisms in a quantity sufficient for their processing. For the first time, this novel emerse photobioreactor strikes a new path to access the metabolite spectrum of terrestrial cyanobacteria. The aim of the applied second project period is 1) to validate the developed analytics, 2) to optimize the production of cyanobacterial valuables by computer aided methods, as well as 3) to scale-up the emerse photobioreactor about a factor of ten. Apart from balancing the mass flow, the project aims to model the flow dynamics in the bioreactor. Altogether, this will allow the final characterization of this novel emerse photobioreactor.This developed photobioreactor is ideally to satisfy the cultivation requirements of terrestrial cyanobacteria. On the one hand, the fibre optics (glass rods), enclosed in the reactor, serves as colonizing surfaces for the organisms, and on the other hand the adherent growing organisms and their growth zones are optimally exposed to the supplied light. Moreover, the supplier of aerosol, integrated at the periphery of the reactor, provides water and nutrients enabling a nutrient-poor cultivation of the target microorganisms. The established geometry as well as the process control permit a cultivation which is highly adjusted to the production of valuables from terrestrial cyanobacteria. In this regard, the experimental set-up facilitates the induced production of specific valuables such as the pigment scytonemin by applying UV-A-radiation and desiccation as well as nitrogen depletion. Moreover, the optimization of exopolysaccharide production is forced, while its composition as well as its content will be modified by varying the supply of aerosol.

陆生蓝藻的特点是有各种各样的贵重物品，这与水生生物有根本的不同。目前，由于栽培技术尚不成熟，这些贵重物品的开发和大量代谢物的生产还没有实现。因此，在项目的第一阶段，我们开发了一种新型的紧急光生物反应器，以培养足够数量的生物来处理它们。这种新型的光生物反应器首次开辟了一条获取陆地蓝藻代谢产物光谱的新途径。应用的第二个项目阶段的目标是1)验证开发的分析方法，2)利用计算机辅助方法优化蓝藻贵重物质的生产，以及3)将紧急光生物反应器放大约10倍。除了平衡质量流，该项目的目标是对生物反应器中的流动动力学进行建模。总之，这将使这种新出现的光生物反应器的最终表征成为可能。这种开发的光生物反应器是满足陆地蓝藻培养要求的理想选择。一方面，封闭在反应器中的光纤(玻璃棒)作为生物体的定植表面，另一方面，附着的生长有机体及其生长区最佳地暴露在所提供的光线下。此外，气雾剂供应商集成在反应堆的外围，提供水和营养物质，使目标微生物能够在营养不足的情况下进行培养。已建立的几何结构和过程控制允许高度调整的栽培，以从陆地蓝藻生产贵重物品。在这方面，该实验装置通过应用UV-A辐射和干燥以及氮气消耗，促进了特定贵重物品的诱导生产，如颜料Sytonmin。此外，胞外多糖生产的优化是被迫的，而其组成和含量将通过改变气雾剂的供应来改变。

项目成果

Characterization of terrestrial cyanobacteria to increase process efficiency in low energy consuming production processes

陆生蓝藻的表征以提高低能耗生产过程中的过程效率

• DOI: 10.1186/2043-7129-1-6
• 发表时间: 2013
• 期刊: Sustainable Chemical Processes
• 作者: Kuhne S;Lakatos M;Foltz S;Muffler K
• 通讯作者: Muffler K