SBIR Phase I: New Convergent X-Ray Beam Based System for Protein Crystallography

0214720Wong这个小型企业创新研究第一阶段项目计划开发一种使用会聚光束的蛋白质X射线结晶学新方法。这项新技术是基于会聚X射线光学和蛋白质二维会聚X射线衍射理论的发展。与传统的准直光学系统相比，会聚光束光学系统可以为X射线光束提供高数量级的通量密度，这对于利用低功率的实验室X射线源测量小的蛋白质晶体(小于100微米)的结构至关重要。在一期工程中，将利用新的软件包CBMPRO生成不同蛋白质晶体的模拟衍射图，从理论上评估蛋白质晶体质量、晶胞、空间群和结构测定的可行性。该项目在蛋白质组学领域的商业应用。

0214720HuangThis Small Business Innovative Research Phase I project proposesto develop a new approach to protein x-ray crystallography using convergent beams. This new technology is based on the development of convergent beam x-ray optics and of protein diffraction theory with 2-dimensional convergent x-ray beams. Compared with conventional collimating optics, convergent beam optics can provide x-ray beams with orders of magnitude higher flux density, which is crucial for structure measurements with small protein crystals (less than 100 um) using low-power laboratory-based x-ray sources. In this Phase I project, simulation diffraction patterns for different protein crystals will be generated by a new software package CBMPRO to theoretically evaluate the feasibility for determination of protein crystal quality, unit cell, space group, and structure.The commercial application of this project is in the area of proteomics.