SBIR Phase I: New Convergent X-Ray Beam Based System for Protein Crystallography

这个小企业创新研究第一阶段项目提议开发一种使用会聚光束的蛋白质x射线晶体学的新方法。这种新技术是基于会聚束x射线光学的发展和二维会聚x射线光束的蛋白质衍射理论。与传统准直光学相比，会聚光束光学可以提供高数量级的通量密度的x射线光束，这对于使用低功率实验室x射线源对小蛋白质晶体（小于100 um）进行结构测量至关重要。在本一期项目中，将使用新的软件包CBMPRO生成不同蛋白质晶体的模拟衍射图，从理论上评估确定蛋白质晶体质量、单位细胞、空间群和结构的可行性。该项目的商业应用是在蛋白质组学领域。

0214720HuangThis Small Business Innovative Research Phase I project proposesto develop a new approach to protein x-ray crystallography using convergent beams. This new technology is based on the development of convergent beam x-ray optics and of protein diffraction theory with 2-dimensional convergent x-ray beams. Compared with conventional collimating optics, convergent beam optics can provide x-ray beams with orders of magnitude higher flux density, which is crucial for structure measurements with small protein crystals (less than 100 um) using low-power laboratory-based x-ray sources. In this Phase I project, simulation diffraction patterns for different protein crystals will be generated by a new software package CBMPRO to theoretically evaluate the feasibility for determination of protein crystal quality, unit cell, space group, and structure.The commercial application of this project is in the area of proteomics.