MRI/RUI: Acquisition of Instrumentation for High Resolution Light Microscopy and Image Processing at Mount Holyoke College

建议6113720PI：Sue Ellen F.Gruber获得芒特霍利奥克学院高分辨率光学显微镜和图像处理仪器摘要授予芒特霍利奥克学院Sue Ellen F.Gruber博士和其他五名生物学家，以获得高分辨率光学显微镜和图像处理仪器。光学显微镜是这些教师和他们在生物系的本科生正在调查的各种生物学问题分析的核心。将获得的仪器，包括与高分辨率冷却CCD摄像机连接的研究级荧光显微镜以及适合图像处理的计算机硬件和软件，将增加这些研究人员的研究需求，并在每种情况下都将允许进行现有设备无法进行的实验和分析。同时，这笔资金将加强生物系以及生物化学、神经科学和行为两个相关专业的学生的研究培训。新仪器将成为综合显微镜设施的一部分，将补充现有的电子显微镜，在几种光学显微镜模式下具有最新的复杂性。六名参与研究的生物学教员正在研究的项目范围很广。其中一项研究是研究影响妊娠成功的母亲和胎儿之间的免疫相互作用。第二个研究探讨了抗疟疾药物在微生物中的作用。第三个是北美独一无二的，旨在记录被忽视的无脊椎动物群体的生物多样性。剩下的项目剖析了导致植物细胞程序性死亡的事件，找出了可能导致小鼠艾滋病相关免疫缺陷的细胞表面特征的身份，并研究了类固醇激素在果蝇发育中的作用。在所有情况下，获得和处理高质量显微镜图像的能力的增强将开辟新的实验途径。芒特霍利奥克学院是一所致力于培养女性成为生产力社会每一个部分的领导者的机构，在培养女科学家方面有着特别出色的记录。它的使命是通过与注重使用现代设备进行实践研究的有成就的教师合作，培养学生作为实践科学家的思维和行为。因此，新仪器的好处将超越特定的教员研究成就，因为它们培养了一代未来科学家的研究培训。

Proposal 6113720PI: Sue Ellen F. GruberAcquisition of Instrumentation for High Resolution Light Microscopy and Image Processing at Mount Holyoke CollegeABSTRACTA grant has been awarded to Dr. Sue Ellen F. Gruber and five other biologists at Mount Holyoke College to acquire instrumentation for high resolution light microscopy and image processing. Light microscopy is core to the analysis of diverse biological questions under investigation by these faculty and their undergraduates in the Biology Department. The instruments to be obtained, including a research-quality fluorescence microscope coupled to a high resolution cooled CCD camera and computer hardware and software appropriate for image processing, will augment the research needs of these researchers, and in each case will permit experimentation and analysis not possible with existing equipment. This grant will, at the same time, enhance the research training of students in the Biology Department, as well as in the two allied programs of Biochemistry, and Neuroscience and Behavior. The new instrumentation, to become part of an integrated microscopy facility, will complement the existing electron microscopes with up-to-date sophistication in the several modes of light microscopy.Projects under investigation by the six participating biology faculty members are wide-ranging. One examines the immunological interactions between a mother and fetus that influence the success of pregnancy. A second explores the action of antimalarial drugs in microorganisms. A third, unique in North America, aims to document the biodiversity of neglected invertebrate groups. The remaining projects dissect the events giving rise to programmed cell death in plants, sort out the identity of cell surface features that may be responsible for AIDS-related immunodeficiency in mice, and examine the role of steroid hormones in the development of fruitflies. In all instances, the enhanced ability to obtain and process high quality microscope images will open up new avenues of experimentation.Mount Holyoke College is an institution dedicated to preparing women to be leaders in every part of productive society and has an especially outstanding record in training women scientists. Its mission is to train students to think and act as practicing scientists, by working with accomplished faculty who emphasize hands-on research with modern equipment. Thus, the benefits of the new instrumentation will extend beyond the particular faculty research accomplishments, as they nurture the research training of a generation of future scientists.