Quantitative continuous single cell analyses by flow cytometry and video-microscopy-based cell tracking (Z01)

通过流式细胞术和基于视频显微镜的细胞追踪进行定量连续单细胞分析 (Z01)

• 批准号: 159847491
• 金额: --
• 依托单位: Klinik 2 - Hämatologie/Onkologie, Rheumatologie und Infektiologie/HIV
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/159847491?language=en
• 关键词: Quantitative; continuous; single; cell; analyses

This project is an extension of the competence centre for multi-parameter flow cytometry and cell sorting (FACS) and video-microscopy-based cell tracking. We will integrate single cell technologies that facilitate the investigation of the function, molecular composition and behaviour of individual cells, using flow-cytometry, single cell sequencing and sensitive protein detection methods. The combination of these technologies bridges the prospective isolation of marker-defined primary endothelial cells at functional and molecular level to unravel molecular cues governing their cell fate decisions in real time.

该项目是多参数流式细胞术和细胞分选 (FACS) 以及基于视频显微镜的细胞跟踪能力中心的延伸。我们将利用流式细胞术、单细胞测序和敏感的蛋白质检测方法，整合单细胞技术，促进单个细胞的功能、分子组成和行为的研究。这些技术的结合在功能和分子水平上连接了标记定义的原代内皮细胞的前瞻性分离，以实时揭示控制其细胞命运决定的分子线索。