Quantitative continuous single cell analyses by flow cytometry and video-microscopy-based cell tracking (Z01)

该项目是多参数流式细胞术和细胞分选（FACS）以及基于视频显微镜的细胞跟踪能力中心的延伸。我们将整合单细胞技术，利用流式细胞术、单细胞测序和敏感蛋白检测方法，促进对单个细胞功能、分子组成和行为的研究。这些技术的结合在功能和分子水平上为标记定义的原代内皮细胞的分离提供了桥梁，以实时揭示控制其细胞命运决定的分子线索。

This project is an extension of the competence centre for multi-parameter flow cytometry and cell sorting (FACS) and video-microscopy-based cell tracking. We will integrate single cell technologies that facilitate the investigation of the function, molecular composition and behaviour of individual cells, using flow-cytometry, single cell sequencing and sensitive protein detection methods. The combination of these technologies bridges the prospective isolation of marker-defined primary endothelial cells at functional and molecular level to unravel molecular cues governing their cell fate decisions in real time.