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Molecular Biology Core

分子生物学核心

基本信息

批准号：
10628216
负责人：
LIN LIU
金额：
$ 23.94万
依托单位：
OKLAHOMA STATE UNIVERSITY STILLWATER
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-07-05 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10628216
关键词：
2019-nCoV Adenovirus Vector Adopted Award Bacteria Biological Assay Bronchoalveolar Lavage CRISPR interference CRISPR screen CRISPR-mediated transcriptional activation CRISPR/Cas technology Cell Line Cells Clinical Clustered Regularly Interspaced Short Palindromic Repeats Communicable Diseases Consultations DNA Microarray Chip Development Epithelial Cells Gene Silencing Generations Genes Human In Vitro Infectious Diseases Research Institution Knock-out Lentivirus Vector Lung Maintenance Methods Microarray Analysis Molecular Molecular Biology Mutagenesis Nasal Epithelium Non-Viral Vector Oklahoma Organoids Pathway interactions Patients Peripheral Blood Mononuclear Cell Phase RNA Interference Reagent Reporter Research Research Personnel Resources Respiratory Center Respiratory Disease Sampling Services Structure of parenchyma of lung Technical Expertise Techniques Technology Text Time Tissue Model Training Transgenes Transgenic Organisms Virus airway epithelium continuous cell line digital gain of function gene transfer vector genome wide screen genome-wide in vivo loss of function overexpression respiratory respiratory pathogen respiratory virus single-cell RNA sequencing transcriptome sequencing transgene expression

项目摘要

Molecular Biology Core Project Summary Molecular biology approaches are utilized to understand the mechanisms underlying respiratory diseases caused by respiratory pathogens. The overall objective of the Molecular Biology Core (MBC) is to provide centralized services and technical expertise in molecular biology for Phase III CoBRE and non-CoBRE investigators. Under the Phase I CoBRE award, the MBC was established to provide services involving molecular biology technologies, including RNAi gene silencing, transgene expression, mutagenesis, gene and pathway reporter assays, real-time PCR, and DNA microarray analyses for gene profiling. State-of-the-art technologies and other services based on the needs of the CoBRE investigators have been continuously developed and added to the Core since its inception. In Phase II, the MBC was expanded to include new techniques, including CRISPR/Cas9 technology and bulk RNA sequencing, and to offer reagents for respiratory and infectious disease research, including cell lines, primary lung cells, and respiratory viruses and bacteria. This Phase III application describes additional cutting-edge technologies and valuable reagents that will be offered to Center investigators, including single-cell RNA sequencing, spatial gene profiling, genome- wide CRISPR screening, human lung tissue models (lung organoids) and clinical samples from respiratory infectious disease patients. The specific aims of the MBC are to provide services and support for 1) functional studies using RNAi and CRISPR/Cas9, transgene overexpression, mutagenesis and deletion, lentiviral, adenoviral and nonviral vectors for gene transfer, and stable knockout and transgenic cell lines, 2) genome- wide gene profiling using bulk RNA sequencing, single-cell RNA sequencing, and spatial gene profiling, 3) genome-wide CRISPR gene activation, knockout and interference screens, 4) molecular assays including reporter assays, real-time PCR and droplet digital PCR, 5) reagents for respiratory and infectious disease research including respiratory viruses and bacteria, and reporter viruses, cell lines, primary lung cells, human lung organoids and clinical samples from respiratory infectious disease patients, and 6) advice, consultation and training on various aspects of molecular biology, developing or adapting new methods as needed and disseminating core resources and technologies.

分子生物学核心