Chlamydomonas genomics: Photosynthesis and acclimation

衣藻基因组学：光合作用和驯化

• 批准号: 0235878
• 负责人: Arthur Grossman
• 金额: $ 316.67万
• 依托单位: Carnegie Institution of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0235878&HistoricalAwards=false
• 关键词: Chlamydomonas; genomics; Photosynthesis; acclimation

This award provides continuing support for the development and application of genomic resources for the study of fundamental biological processes in Chlamydomonas, an important model organism. This project will enhance the use of Chlamydomonas for elucidating the regulation of the photosynthetic apparatus with respect to changing environmental conditions. The investigators have the following five goals: (1) Implement a Generic Model Organism Database (GMOD) for Chlamydomonas that will display the genome and its features, including coding regions, ESTs, cDNAs, splice sites, BAC positions, molecular markers and sites of insertion elements in mutant strains. (2) Create a Phase II microarray representing 8,500-10,000 unique genes and supplement the array with an oligonucleotide-based array that will include genes that are defined by whole genome sequencing and that are not included on the Phase II array. Arrays will be used to catalog mechanisms of acclimation to environmental stress, using both wild-type and mutant strains whose biological phenotypes have already been determined; an emphasis will be placed on photosynthetic function. (3) Build an insertional mutant collection saturated for nuclear genes whose products are linked to photosynthetic function. Insertion strains will be challenged with a battery of screens, and the insertion sites of 5,000 strains affected in photosynthetic function will be sequenced and made available to the community. (4) Create high-throughput mapping tools to facilitate gene isolation from mutants that are not tagged by insertions. These will primarily be suppressors of other mutations, or mutations in genes for which a null lesion would be lethal. The mapping will be based on simple sequence repeats, automated and made more rapid by the use of bulked segregant analysis. A mapping service will also be offered to the community.(5) Develop vectors to facilitate RNA interference-mediated reverse genetics.Chlamydomonas reinhardtii, a unicellular haploid green alga, is an important model system for elucidating basic biological processes, especially in plants. The further development of sophisticated genome-wide methodologies for studies of Chlamydomonas will greatly augment its usefulness to the scientific community. This project will also produce extensive data on photosynthesis and acclimation, adding to our knowledge of how photosynthetic organisms sense, respond to, and survive in a rapidly changing environment. It will also enhance Chlamydomonas's usefulness as a 'training' organism in laboratories throughout the world, and provide a new and exciting entry point into research careers for young scientists.

该奖项为衣原体（一种重要的模式生物）基本生物学过程研究的基因组资源的开发和应用提供了持续的支持。 这个项目将加强利用衣原体阐明光合机构的调节与不断变化的环境条件。 研究人员有以下五个目标：（1）建立一个通用模式生物数据库（GMOD），该数据库将显示衣原体的基因组及其特征，包括编码区、EST、cDNA、剪接位点、BAC位置、分子标记和突变株中插入元件的位点。(2)创建一个代表8，500 - 10，000个独特基因的II期微阵列，并使用基于谷胱甘肽的阵列补充该阵列，该阵列将包括通过全基因组测序定义的基因，并且不包括在II期阵列中。 阵列将被用来编目机制的驯化环境压力，使用野生型和突变株的生物表型已经确定，重点将放在光合功能。(3)建立一个插入突变体收集饱和的核基因，其产品与光合功能。插入菌株将受到一系列筛选的挑战，5，000株光合功能受影响的菌株的插入位点将被测序并提供给社区。 (4)创建高通量作图工具，以促进从未通过插入标记的突变体中分离基因。这些将主要是其他突变的抑制因子，或基因突变，无效病变将是致命的。作图将基于简单的重复序列，自动化，并通过使用混合分离分析更快速。还将向社区提供地图服务。(5)莱茵衣藻是一种单细胞单倍体绿色植物，是研究植物基本生物学过程的重要模式系统。进一步发展复杂的全基因组方法来研究衣原体，将大大增加其对科学界的用处。 该项目还将产生关于光合作用和适应的广泛数据，增加我们对光合生物如何感知，响应和在快速变化的环境中生存的知识。它还将提高衣原体作为世界各地实验室中“训练”有机体的实用性，并为年轻科学家的研究生涯提供一个新的令人兴奋的切入点。