CAREER: Deoxyribozyme-Deoxyribozyme Logic, a New Computational Substrate

职业：脱氧核酶-脱氧核酶逻辑，一种新的计算基质

• 批准号: 0238027
• 负责人: Darko Stefanovic
• 金额: --
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0238027&HistoricalAwards=false
• 关键词: CAREER; Deoxyribozyme; Logic; New; Computational

EIA-0243796Darko StefanovicUniversity of New MexicoDeoxyribozyme-deoxyribozyme Logic, a new computational substrateDeoxyribozymes (nucleic acid enzymes) are the basis for a family of molecular-scale elementary logic gates which have input and output signals of the same kind, as concentrations of oligonucleotides, and which operate in solution. Chemical-kinetic properties of these gates are being studied through collaborative experiments, analytical modelling, and computer simulation. Dynamic properties of larger circuits composed of elementary gates and capable of autonomously carrying out complex Boolean functions (decision-making) as well as other computational tasks are being studied, including the reliability of communication between gates, in order to characterize the functional behavior and performance of such circuits. Analysis and synthesis tools tailored to deoxyribozyme-based logic are being developed.Through this work, the state of knowledge about large-scale chemical kinetics modelling, oligonucleotide library design and other combinatorial problems is being advanced, and interdisciplinary collaborations between computer science and biochemistry and nanotechnology are being established. Through involvement in research and through curriculum development, undergraduate and graduate students are being trained in the emerging area of bioinformatics. Finally, foundations are being laid for diagnostic and therapeutic applications in which individual cells can be analyzed according to Boolean criteria, so that diseased cells can be reliably distinguished from healthy ones and specifically treated.

脱氧核酶-脱氧核酶逻辑是一种新的计算底物脱氧核酶(核酸酶)是一系列分子规模基本逻辑门的基础，这些门具有与寡核苷酸浓度相同的输入和输出信号，并在溶液中运行。这些门的化学动力学特性正在通过合作实验、分析模型和计算机模拟进行研究。正在研究由基本门组成并能够自主执行复杂布尔函数(决策)以及其他计算任务的较大电路的动态特性，包括门之间通信的可靠性，以便表征这种电路的功能行为和性能。为脱氧核酶逻辑量身定做的分析和合成工具正在开发中，通过这项工作，大规模化学动力学建模、寡核苷酸文库设计和其他组合问题的知识状况正在提升，计算机科学与生物化学和纳米技术之间的跨学科合作正在建立。通过参与研究和课程开发，本科生和研究生正在接受生物信息学这一新兴领域的培训。最后，正在为诊断和治疗应用奠定基础，在这些应用中，可以根据布尔标准分析单个细胞，以便可以可靠地将患病细胞与健康细胞区分开来并进行专门治疗。