CAREER: Pathways to in situ Medication: Liposomal Encapsulation, Transport in Microchannels and Delivery via Liposomal Membrane Crumpling

职业：原位药物治疗途径：脂质体封装、微通道运输以及通过脂质体膜压皱输送

• 批准号: 0238874
• 负责人: Sahraoui Chaieb
• 金额: --
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-15 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0238874&HistoricalAwards=false
• 关键词: CAREER; Pathways; situ; Medication; Liposomal

Abstract:The main treatments of tumoral diseases such as cancer are radiation, which can be damaging to healthy organs or surgery, which can be disfiguring. While with chemotherapy only a small fraction of the administered dose reaches the site whilst the rest is distributed throughout the body which limits the dosage and prevents the method from achieving its function of curing. The objective of the proposed research is to develop and study a system whereby cell-like objects (liposomes) are filled with a drug, transported in microchannels and are induced, by temperature, to deliver the payload at precise locations. The project involves experimental and theoretical work at the forefront of research on the behavior of biological materials where solid mechanics, fluid mechanics and material sciences meet. This will be a cooperative effort with the diagnostic group of the Hospital Products Division at Abbott Laboratories.This problem will necessitate the creation of new directions in mechanical sciences to treat various cases of deformation of liposomes: Instability-induced deformation of large tubes to create small spherical vesicles. Flow induced deformations during the transport of drug-laden liposomes and temperature-induced deformations during the delivery of the drug. These ideas lay in the coupling of elasticity with hydrodynamics. Experimentally we will use fluorescence microscopy to monitor the local state of the liposomes and we will use nano-indentation to probe mechanical properties of the liposomes. Techniques in microfibration will be developed to study the transport of these objects in microchannels.This research project will be integrated with a curriculum being developed at the University of Illinois at Urbana-Champaign such as the newly developed graduate program in bioengineering and the secondary option of biomechanics newly introduced by the PI in the department of mechanics. The main interest of this course is the study of cell and tissue mechanics where biologists are exposed to engineering methods and engineering students are exposed to protocols of cell biology.

翻译后摘要：肿瘤疾病，如癌症的主要治疗方法是辐射，这可能会损害健康的器官或手术，这可能会毁容。 而对于化疗，只有一小部分给药剂量到达该部位，而其余部分分布在全身，这限制了剂量并阻止了该方法实现其治愈功能。拟议研究的目的是开发和研究一种系统，使细胞样物体（脂质体）充满药物，在微通道中运输，并通过温度诱导，将有效载荷传递到精确的位置。该项目涉及固体力学，流体力学和材料科学相遇的生物材料行为研究前沿的实验和理论工作。这将是与Abbott实验室医院产品部诊断小组的合作努力。这个问题将需要在机械科学中创造新的方向来处理脂质体变形的各种情况：不稳定性引起的大管变形以产生小的球形囊泡。载药脂质体运输期间的流动诱导变形和药物递送期间的温度诱导变形。这些想法在于弹性与流体力学的耦合。在实验上，我们将使用荧光显微镜来监测脂质体的局部状态，我们将使用纳米压痕来探测脂质体的机械性质。将开发微纤维化技术来研究这些物体在微通道中的传输。该研究项目将与伊利诺伊大学厄巴纳-香槟分校正在开发的课程相结合，例如新开发的生物工程研究生课程和PI在力学系新引入的生物力学第二选项。本课程的主要兴趣是细胞和组织力学的研究，生物学家接触工程方法，工程专业的学生接触细胞生物学协议。