COLLABORATIVE RESEARCH. The Role of Meso- and Bathypelagic Prokaryotic Organisms in the Marine Water Column: Insights from Compound-Specific Radiocarbon Analysis
合作研究。
基本信息
- 批准号:0241363
- 负责人:
- 金额:$ 22.62万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2003
- 资助国家:美国
- 起止时间:2003-03-01 至 2006-01-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
ABSTRACTOCE-0242160OCE-0241363Even though the meso- and bathypelagic regions beneath the euphotic zone contain nearly all of the oceans volume, little is known about the biogeochemical processes that function in this region. For this reason, a PI from Scripps Institute of Oceanography will collaborate with a PI from Harvard University to determine which carbon sources fuel prokaryotic production in the meso- and bathypelagic ocean. Specifically, the PIs wish to address the following two questions: (1) Is freshly-produced organic matter the primary dissolved organic carbon (DOC) pool that is accessible to deep ocean heterotrophic prokaryotes or is "old" DOC also bioavailable?; and (2) Is chemoautotrophic fixation of inorganic carbon by planktonic archaea an important component of the deep ocean carbon cycle? To address these questions, the PIs plan to measure the radiocarbon (14C) concentration of the carbon source pools and the resulting prolaryotic (bacterial and archaeal) biomass at the surface, meso (500-600 m) and bathypelagic (900 m) depths. In addition, fluorescent in-situ hybridization (FISH) and genetic diversity analysis by denaturing gradient gel electrophorsis (DGGE) will provide a complementary picture of the biological community. Lastly, to obtain the large quantities of biomass-derived carbon needed for this study, the PIs plan to develop an in-situ, battery powered, submersible pump capable of pumping 20,000L of seawater per deployment during the first year of the study.
尽管真光层下面的中层和深海区域包含了几乎所有的海洋体积,但人们对该区域发挥作用的生物地球化学过程知之甚少。出于这个原因,斯克里普斯海洋研究所的一名PI将与哈佛大学的一名PI合作,以确定哪些碳源为中、深海的原核生物生产提供燃料。具体而言,PIs希望解决以下两个问题:(1)新鲜产生的有机物质是深海异养原核生物可获得的主要溶解有机碳(DOC)池,还是“老的”DOC也可生物利用?(2)浮游古细菌对无机碳的化学自养固定是深海碳循环的重要组成部分吗?为解决这些问题,私营部门计划测量碳源库的放射性碳(14C)浓度,以及由此产生的表层、中层(500-600米)和深海(900米)的前节肢动物(细菌和古生物)生物量。此外,荧光原位杂交(FISH)和变性梯度凝胶电泳(DGGE)遗传多样性分析将提供生物群落的互补图景。最后,为了获得这项研究所需的大量生物质碳,PIS计划在研究的第一年开发一种原地电池供电的潜水泵,每次部署能够泵送20,000升海水。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ann Pearson其他文献
Who lives in the sea floor?
谁住在海底?
- DOI:
10.1038/454952a - 发表时间:
2008-08-20 - 期刊:
- 影响因子:48.500
- 作者:
Ann Pearson - 通讯作者:
Ann Pearson
Stable carbon isotope ratios of pristine carbohydrates preserved within nannofossil calcite
- DOI:
10.1016/j.gca.2024.09.007 - 发表时间:
2025-01-01 - 期刊:
- 影响因子:
- 作者:
Harry-Luke Oliver McClelland;Renee B.Y. Lee;Ann Pearson;Rosalind E.M. Rickaby - 通讯作者:
Rosalind E.M. Rickaby
Metabolic imprints in the hydrogen isotopes of <em>Archaeoglobus fulgidus</em> tetraether lipids
- DOI:
10.1016/j.gca.2024.09.032 - 发表时间:
2024-12-01 - 期刊:
- 影响因子:
- 作者:
Jeemin H. Rhim;Sebastian Kopf;Jamie McFarlin;Ashley E. Maloney;Harpreet Batther;Carolynn M. Harris;Alice Zhou;Xiahong Feng;Yuki Weber;Shelley Hoeft-McCann;Ann Pearson;William D. Leavitt - 通讯作者:
William D. Leavitt
Metabolic imprints in the hydrogen isotopes of Archaeoglobus fulgidus tetraether lipids
古生球菌四醚脂质氢同位素的代谢印记
- DOI:
10.1101/2023.11.29.569324 - 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Jeemin H. Rhim;Sebastian H. Kopf;Jamie McFarlin;Ashley E Maloney;H. Batther;Carolynn M. Harris;A. Zhou;Xiahong Feng;Yuki Weber;S. Hoeft;Ann Pearson;W. Leavitt - 通讯作者:
W. Leavitt
12 month re-referral rates of 710 patients attending a low-risk breast clinic
12 个月对 710 名参加低风险乳腺癌诊所的患者的再次转诊率
- DOI:
10.1016/j.ejso.2024.108161 - 发表时间:
2024-05-01 - 期刊:
- 影响因子:2.900
- 作者:
Libby Doughty;Sita Riley;Samantha Ng;Ann Pearson;Sophia Sakellariou;James Mansell;Julie Trafford;Jennifer Campbell - 通讯作者:
Jennifer Campbell
Ann Pearson的其他文献
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{{ truncateString('Ann Pearson', 18)}}的其他基金
Collaborative Research: Illuminating the Cenozoic Alkenone pCO2 Record
合作研究:阐明新生代烯酮 pCO2 记录
- 批准号:
2100537 - 财政年份:2021
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
"Carbon isotope fractionation in Archaea using the 3HP/4HB pathway: Prospects for paleo-geochemistry and paleo-barometry"
“使用 3HP/4HB 途径进行古生菌碳同位素分馏:古地球化学和古气压测量的前景”
- 批准号:
1843285 - 财政年份:2019
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
Beyond ocean temperature: Extracting new dimensions of paleoclimatic information from archaeal lipids and their isotopic compositions
超越海洋温度:从古细菌脂质及其同位素组成中提取新维度的古气候信息
- 批准号:
1702262 - 财政年份:2017
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
A window on ancient systems: Understanding the environmental distributions of hopanoids using compound-specific multi-isotope analysis and metagenomics
了解古代系统的窗口:使用化合物特异性多同位素分析和宏基因组学了解藿类化合物的环境分布
- 批准号:
1349126 - 财政年份:2014
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
Pure standards of archaeal and bacterial GDGTs for the oceanographic community
海洋学界古细菌和细菌 GDGT 的纯标准
- 批准号:
1129343 - 财政年份:2011
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
Dimensions: Collaborative Research: Functional Diversity of Microbial Trophic Guilds Defined Using Stable Isotope Ratios of Proteins
维度:合作研究:使用蛋白质稳定同位素比率定义的微生物营养组的功能多样性
- 批准号:
1136484 - 财政年份:2011
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
Carbon isotopic heterogeneity in modern bacterioplankton: Model systems for understanding paleoenvironmental signatures.
现代浮游细菌中的碳同位素异质性:用于理解古环境特征的模型系统。
- 批准号:
0927290 - 财政年份:2009
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
"Collaborative Research: Constraining the marine nitrogen cycle using a new approach to measuring nitrogen isotopes of chlorins"
“合作研究:使用测量二氢卟酚氮同位素的新方法限制海洋氮循环”
- 批准号:
0825269 - 财政年份:2008
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
Environmental Phylogeny, Physiology, and Evolutionary Significance of Polycyclic Triterpenoids
多环三萜类化合物的环境系统发育、生理学和进化意义
- 批准号:
0641899 - 财政年份:2007
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
A New Interdisciplinary Frontier: Merging Molecular Biology with Isotopic Geochemistry
新的跨学科前沿:分子生物学与同位素地球化学的融合
- 批准号:
0311937 - 财政年份:2003
- 资助金额:
$ 22.62万 - 项目类别:
Standard Grant
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