Dynamic of axonal activity during fast network oscillations and plasticity

快速网络振荡和可塑性期间轴突活动的动力学

• 批准号: 161844901
• 负责人: Professor Dr. Tengis Gloveli
• 金额: --
• 依托单位: Neurowissenschaftliches Forschungszentrum (CCM)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161844901?language=en
• 关键词: Dynamic; axonal; activity; during; fast

In hippocampal pyramidal cells, as in other neurons, initiation of action potentials (APs) occurs in the axon close to the soma. APs can also be generated at regions remote from the soma, at ectopic sites of the axon, as an emergent intrinsic property of the neuronal network. We will examine the possibility that such ectopically generated APs (EAPs) may be initiated at presynaptic axon terminals of principal cells of the hippocampus during normal physiological network activity such as gamma frequency oscillations. Parvalbumin containing perisomatic targeting axo-axonic cells (AACs) form synapses at strategic sites of pyramidal cells, namely at the axon initial segment (AIS). We will investigate the possibility that this cell type could routinely confine the propagation of EAPs to the soma during the gamma oscillations and therefore may have critical impact on the excitability of principal cells. Paired recordings will show whether activation of presynaptic AACs results in suppression of EAPs invasion to the parent soma of monosynaptically coupled principal cells. We will further examine whether suppression of AP back-propagation is mediated through a membrane potential hyperpolarization/ depolarization or a shunting inhibition. We hypothesise that the modulation/suppression of spike backpropagation along the pyramidal cell axon by AACs allows axons to fire independently from their somata and to transmit their message to the postsynaptic targets, whilst at the same time to spare the complex dendritic integration during relevant physiological network activity.

在海马锥体细胞中，与其他神经元一样，动作电位（AP）的起始发生在靠近索马体的轴突中。AP也可以在远离索马的区域产生，在轴突的异位部位，作为神经元网络的涌现的内在特性。我们将研究的可能性，这种异位产生的AP（EAP）可能会启动在突触前轴突终端的海马的主细胞在正常的生理网络活动，如伽马频率振荡。含有小清蛋白的体周靶向轴-轴细胞（AAC）在锥体细胞的战略位点，即在轴突起始段（AIS）处形成突触。我们将调查的可能性，这种细胞类型可以常规限制传播的EAP的索马在伽玛振荡，因此可能有关键的影响，对主细胞的兴奋性。配对记录将显示突触前AAC的激活是否导致EAP侵入单突触耦合主细胞的亲本索马的抑制。我们将进一步研究AP反向传播的抑制是否通过膜电位超极化/去极化或分流抑制介导。我们假设，调制/抑制尖峰反向传播沿着的锥体细胞轴突的AAC允许轴突火独立于他们的胞体和传递他们的信息到突触后的目标，而在同一时间，以节省相关的生理网络活动过程中的复杂的树突整合。

项目成果

Cell Type-Specific Separation of Subicular Principal Neurons during Network Activities

• DOI: 10.1371/journal.pone.0123636
• 发表时间: 2015-04-14
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Eller, Joanna;Zarnadze, Shota;Gloveli, Tengis
• 通讯作者: Gloveli, Tengis

Cell-specific synaptic plasticity induced by network oscillations

网络振荡诱导的细胞特异性突触可塑性

• DOI: 10.7554/elife.14912
• 发表时间: 2016
• 期刊: eLife
• 影响因子: 7.7
• 作者: Zarnadze S;Bäuerle P;Santos-Torres J;Böhm C;Schmitz D;Geiger JR;Dugladze T;Gloveli T
• 通讯作者: Gloveli T

GABAB autoreceptor-mediated cell type-specific reduction of inhibition in epileptic mice

• DOI: 10.1073/pnas.1313505110
• 发表时间: 2013-09-10
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Dugladze, Tamar;Maziashvili, Nino;Gloveli, Tengis
• 通讯作者: Gloveli, Tengis