International Research Fellowship Program: Genetic Analysis of Arabidopsis Seed Endosperm Development

[0301886]菲茨杰拉德国际研究奖学金计划使美国科学家和工程师能够在国外进行三到二十四个月的研究。该计划的奖励为联合研究提供了机会，并利用独特或互补的设施、专业知识和国外的实验条件。该奖项将支持乔纳森·n·菲茨杰拉德博士与弗雷德里克·伯杰博士在法国里昂高等师范学院开展为期24个月的研究。在动物中，发育胚胎的模式是由Polycomb组（PcG）和Trithorax组（TxG）染色质重塑复合物的活性建立的。在拟南芥中，宿主实验室开发的高分辨率延时3D共聚焦显微镜技术可以可视化PcG在发育种子中的作用。有了这个强大的实验系统，宿主实验室就可以通过这类重要的DNA修饰复合物来研究基因调控和模式。PI计划分离和遗传表征一种新的拟南芥txg样活性，KRONOS，已在宿主实验室中确定。拟议的KRONOS功能研究将进一步加深我们对发育过程中染色质调控的机制理解。

0301886FitzGeraldThe International Research Fellowship Program enables U.S. scientists and engineers to conduct three to twenty-four months of research abroad. The program's awards provide opportunities for joint research, and the use of unique or complementary facilities, expertise and experimental conditions abroad.This award will support a twenty-four month research fellowship by Dr. Jonathan N. FitzGerald to work with Dr. Frederic Berger at Ecole Normale Superieure de Lyon in Lyon, France.In animals, the pattern of the developing embryo is established by the activity of the Polycomb group (PcG) and Trithorax group (TxG) chromatin remodeling complexes. In Arabidopsis thaliana, the high resolution time-lapse 3D confocal microscopy techniques developed in the host lab enable visualization of PcG action in the developing seed. With this powerful experimental system the host lab is poised for studying gene regulation and patterning by this important class of DNA modifying complexes. The PI plans to isolate and genetically characterize a novel Arabidopsis TxG-like activity, KRONOS, that has been identified in the host lab. Proposed functional studies of KRONOS will further our mechanistic understanding of chromatin regulation during development.