RUI-Identification of Genes and Cellular Processes Targeted by Imprinted Pathways in Natural Variants of Arabidopsis thaliana

RUI-拟南芥天然变种中印迹途径靶向的基因和细胞过程的鉴定

• 批准号: 1244101
• 负责人: Jonathan FitzGerald
• 金额: $ 41.25万
• 依托单位: Rhodes College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244101&HistoricalAwards=false
• 关键词: RUI; Identification; Genes; Cellular; Processes

Although it is common knowledge that we receive half of our genes from our mother and half from our fathers, it is less widely known that the genes we inherit can play different roles depending on which parent provided them. For example, genes regulated by parental genomic imprinting (PGI) are silenced either maternally or paternally (depending on the gene) after fertilization. PGI has been characterized in mammals and seed plants, and in both cases has been linked in part to the regulation of maternal resources available to the offspring. Using the plant model Arabidopsis thaliana, it has been found that plants isolated from different regions can promote strong increases in seed size depending on whether they are used as mothers or fathers in crosses. This might further suggest that parental bias for resource allocation, or the even selection of imprinted genes themselves, is subject to variation in natural populations. This project aims to identify the genes involved in these parental effects using recombinant inbred (RI) lines, which are genetic chimeras of two initial parents, and mapping populations that carry visual markers for maternal gene expression. In addition, seed development will be characterized at the cellular level to better describe the developmental outcomes of variation in imprinted or parentally-biased systems. Broader impacts. This project seeks to identify seed size genes that may function in short-term adaptive strategies for genomic regulation, accounting for some trait variation between Arabidopsis ascensions. This aspect is directly translatable to novel breeding strategies in agriculture. This project will also aim to advance education at the boundary of biology and mathematics. The RI lines employed are uniquely suited as a classroom tool to bridge basic genetics and physiology with computational methods. A learning module has been created to conduct publishable RI research in the Plant Genetics and Diversity course at Rhodes College. Resources will be generated from these course materials allowing educators at both the university and high school level to employ RI systems in the classroom. Educational goals will be further supported by involving undergraduate researchers at all stages of this work, and underrepresented minorities in science will continue to be actively recruited for these research projects.Co-funded by the Genetic Mechanisms Cluster in Molecular and Cellular Biosciences and the the Experimental Program to Stimulate Competitive Research.

虽然我们的基因一半来自母亲，一半来自父亲，这是常识，但我们继承的基因可以根据父母提供的不同发挥不同的作用，这一点鲜为人知。 例如，由亲本基因组印记（PGI）调控的基因在受精后在母系或父系（取决于基因）沉默。 PGI的特点是在哺乳动物和种子植物，并在这两种情况下，已被链接到部分调节可供后代的母体资源。 使用植物模型拟南芥，已经发现从不同区域分离的植物可以促进种子大小的强烈增加，这取决于它们在杂交中是用作母本还是父本。 这可能进一步表明，资源分配的父母偏见，甚至选择印迹基因本身，是受自然群体的变化。该项目的目的是使用重组近交（RI）系，这是两个初始的父母，和映射人口携带视觉标记的母系基因表达的遗传嵌合体，以确定这些父母的影响所涉及的基因。 此外，种子发育将在细胞水平上进行表征，以更好地描述印记或亲本偏倚系统中变异的发育结果。更广泛的影响。该项目旨在确定种子大小基因，可能在基因组调控的短期适应性策略中发挥作用，说明拟南芥上升之间的一些性状变异。这方面可以直接转化为农业中的新育种策略。 该项目还旨在推进生物学和数学领域的教育。所采用的RI线是唯一适合作为一个教室工具，以桥梁基础遗传学和生理学与计算方法。在罗兹学院的植物遗传学和多样性课程中，已经创建了一个学习模块来进行可持续的RI研究。这些课程材料将产生资源，使大学和高中教育工作者能够在课堂上使用国际扶轮系统。将通过让本科生研究人员参与这项工作的各个阶段来进一步支持教育目标，并将继续积极招募科学界代表性不足的少数群体参与这些研究项目，由分子和细胞生物科学遗传机制集群和刺激竞争性研究的实验方案共同资助。