U.S.-Mexico Program: Development of Rapid Methods for Plant Membrane Transport Protein Localization Using Epitope Tags

美国-墨西哥项目：开发使用表位标签进行植物膜转运蛋白定位的快速方法

基本信息

批准号：
0305414
负责人：
Daniel Schachtman
金额：
$ 1.93万
依托单位：
Donald Danforth Plant Science Center
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2003
资助国家：
美国
起止时间：
2003-06-01 至 2006-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0305414&HistoricalAwards=false
关键词：
U.S.Mexico Program Development Rapid

项目摘要

0305414SchachtmanThis U.S.-Mexico award will support Dr. Daniel Schachtman of the Donald Danforth Plant Science Center in St. Louis, Missouri, in a research collaboration with Dr. Bronwyn J. Barkla of the Universidad Nacional Autonoma de Mexico (UNAM) in Cuernavaca, Morelos, Mexico. The researchers intend to work on several transport proteins (KUPs) that are involved in potassium uptake and cellular equilibrium. Potassium is an essential mineral for both animal and plant nutrition. Plants require large quantities of this mineral, which they acquire through root uptake from soils. Several families of transporters are involved in moving potassium into cells and around the plant. The KUPs (potassium uptake permeases) are a major family of proteins involved in potassium uptake into cells, a process that drives cell expansion and plant development. The proposed collaboration will focus on methods for the membrane localization of the KUP proteins, an important objective since little is known about which membranes these proteins are located on. The knowledge gained will help to understand their function fully. The collaborators will benefit from the Mexican lab's expertise in plant membrane transporter biochemistry and the St. Louis lab's expertise in plant molecular and cell biology. A postdoctoral fellow and a student will take part in the exchange and will be trained in membrane biochemistry.

0305414SCHACHTMANTHIS US-MEXICO奖将支持密苏里州圣路易斯Donald Danforth Plant Science Center的Daniel Schachtman博士。研究人员打算从事钾摄取和细胞平衡涉及的几种转运蛋白（KUP）。钾是动物和植物营养的必不可少的矿物质。植物需要大量这种矿物，它们通过土壤的根源吸收而获取。几个转运蛋白家族参与将钾转移到细胞和植物周围。 KUPS（钾摄入腐蚀）是参与细胞摄入钾的主要蛋白质家族，这一过程驱动细胞扩张和植物发育。提出的合作将集中于KUP蛋白质膜定位的方法，这是一个重要的目标，因为这些蛋白质所在的膜知之甚少。获得的知识将有助于充分理解其功能。合作者将受益于墨西哥实验室在植物膜转运蛋白生物化学方面的专业知识以及圣路易斯实验室在植物分子和细胞生物学方面的专业知识。博士后研究员和学生将参加交流，并将接受膜生物化学的培训。