U.S.-Mexico Program: Development of Rapid Methods for Plant Membrane Transport Protein Localization Using Epitope Tags

美国-墨西哥项目：开发使用表位标签进行植物膜转运蛋白定位的快速方法

• 批准号: 0305414
• 负责人: Daniel Schachtman
• 金额: $ 1.93万
• 依托单位: Donald Danforth Plant Science Center
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0305414&HistoricalAwards=false
• 关键词: U.S.; Mexico; Program; Development; Rapid

0305414SchachtmanThis U.S.-Mexico award will support Dr. Daniel Schachtman of the Donald Danforth Plant Science Center in St. Louis, Missouri, in a research collaboration with Dr. Bronwyn J. Barkla of the Universidad Nacional Autonoma de Mexico (UNAM) in Cuernavaca, Morelos, Mexico. The researchers intend to work on several transport proteins (KUPs) that are involved in potassium uptake and cellular equilibrium. Potassium is an essential mineral for both animal and plant nutrition. Plants require large quantities of this mineral, which they acquire through root uptake from soils. Several families of transporters are involved in moving potassium into cells and around the plant. The KUPs (potassium uptake permeases) are a major family of proteins involved in potassium uptake into cells, a process that drives cell expansion and plant development. The proposed collaboration will focus on methods for the membrane localization of the KUP proteins, an important objective since little is known about which membranes these proteins are located on. The knowledge gained will help to understand their function fully. The collaborators will benefit from the Mexican lab's expertise in plant membrane transporter biochemistry and the St. Louis lab's expertise in plant molecular and cell biology. A postdoctoral fellow and a student will take part in the exchange and will be trained in membrane biochemistry.

0305414沙赫特曼美国-墨西哥奖将支持密苏里州圣路易斯唐纳德·丹福斯植物科学中心的丹尼尔·沙赫特曼博士与墨西哥国立自治大学（UNAM）的布朗温·J·巴克拉博士（Bronwyn J. Barkla）进行研究合作，该大学位于墨西哥莫雷洛斯的库埃尔纳瓦卡。 研究人员打算研究几种参与钾吸收和细胞平衡的转运蛋白（KUP）。钾是动物和植物营养的必需矿物质。 植物需要大量的这种矿物质，它们通过根从土壤中吸收获得。 几个家族的转运蛋白参与将钾转移到细胞内和植物周围。 KUP（钾吸收渗透酶）是参与细胞钾吸收的主要蛋白质家族，这是一个驱动细胞扩增和植物发育的过程。 拟议的合作将集中在KUP蛋白的膜定位方法上，这是一个重要的目标，因为人们对这些蛋白位于哪种膜上知之甚少。所获得的知识将有助于全面了解它们的功能。合作者将受益于墨西哥实验室在植物膜转运蛋白生物化学方面的专业知识和圣路易斯实验室在植物分子和细胞生物学方面的专业知识。一名博士后研究员和一名学生将参加交流，并将接受膜生物化学方面的培训。