Integration Of Microscopy And Biochemistry Technologies To Enhance Quantitative Experimental Cell Biology Within An Inquiry-Based Curriculum

显微镜和生物化学技术的整合，以增强基于探究的课程中的定量实验细胞生物学

• 批准号: 0310627
• 负责人: Shubhik DebBurman
• 金额: $ 7.09万
• 依托单位: Lake Forest College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0310627&HistoricalAwards=false
• 关键词: Integration; Microscopy; Biochemistry; Technologies; Enhance

Undergraduate educators face important challenges in preparing diverse graduates for a technologically sophisticated and scientifically interdisciplinary community. Lake Forest College Biology Department is meeting this challenge with inquiry-based pedagogy that supports a diverse student-centered learning community. We expect students to develop scientific process and master content by progressing through increasingly sophisticated hypothesis-driven investigations that require collaborative research, critical thinking, and diverse forms of communication. Currently, both courses that anchor our cellular curriculum, introductory Cell Biology and advanced Molecular Genetics, feature DNA-based investigations. Other cellular-level technologies feature prominently in biomedical discoveries. Intellectual Merit: We are creating a more comprehensive cellular curriculum by redressing existing deficiencies in cell culture, microscopy and protein biochemistry. We are adding investigative experiments in these areas by restructuring introductory Cell Biology and adding two more advanced courses. Inquiry-conducive experiments successful at the College of the Holy Cross that require quantitative microscopy of cells and tissues and biochemical analysis of proteins are being adapted and implemented. To implement these changes, instrumentation for cell culturing, histology, microscopy, and biochemical analysis is being acquired. The introductory course incorporates investigations in cell culturing and protein characterization. Advanced course students are expanding their investigative independence and technical repertoire by developinghypotheses based on current literature and conducting investigations featuring cell culturing, histology, fluorescent microscopy, and protein biochemistry. These additions also support other courses and help establish interdisciplinary biochemistry and neuroscience majors. Broader Impact: We aim to graduate diverse individuals thoroughly trained to succeed in a variety of scientific careers, provide innovative K-12 outreach, and nationally disseminate innovations.

本科教育工作者在为技术先进和科学跨学科的社会培养多样化的毕业生方面面临着重要的挑战。森林湖学院生物系以探究教学法迎接这一挑战，支持以学生为中心的多元化学习社区。我们希望学生通过越来越复杂的假设驱动的调查来发展科学的过程和掌握内容，这些调查需要合作研究、批判性思维和多种形式的交流。目前，这两门课程锚定我们的细胞课程，细胞生物学入门和高级分子遗传学，以dna为基础的研究。其他细胞水平的技术在生物医学发现中占有突出地位。智力优势：我们正在创建一个更全面的细胞课程，通过弥补现有的缺陷，细胞培养，显微镜和蛋白质生物化学。我们正在通过重组细胞生物学入门课程和增加两门更高级的课程，在这些领域增加研究性实验。在圣十字学院成功进行的有利于探究的实验，需要对细胞和组织进行定量显微镜观察，并对蛋白质进行生化分析，目前正在进行调整和实施。为了实现这些变化，正在获得用于细胞培养，组织学，显微镜和生化分析的仪器。导论课程包括细胞培养和蛋白质特性的研究。高级课程的学生在现有文献的基础上发展假设，并开展以细胞培养、组织学、荧光显微镜和蛋白质生物化学为特色的调查，从而扩大他们的研究独立性和技术技能。这些补充也支持其他课程，并有助于建立跨学科的生物化学和神经科学专业。更广泛的影响：我们的目标是毕业多样化的个人彻底训练，在各种科学事业中取得成功，提供创新的K-12外展，并在全国范围内传播创新。