A Rice Oligonucleotide Array

水稻寡核苷酸阵列

• 批准号: 0313887
• 负责人: Pamela Ronald
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0313887&HistoricalAwards=false
• 关键词: Rice; Oligonucleotide; Array

Rice has become a model for grasses and cereal because of its small genome size, available genome sequence, and ease of transformation. The structural and functional analysis of rice genes has broad practical implications for the other economically important cereals such as corn and wheat. One approach, microarray analysis, permits biologists to measure the expression levels of thousands of genes in single experiment. Biologically significant information can be extracted from these data computationally, and then used to assign functions to genes, and to identify genetic regulatory networks. In this project, we will:1) Design 40,000 70-mer oligomers and construct microarrays for project use and public distribution. 2) Construct a data analysis system, database and user interface for project data. Develop web-based tools for data mining the rice transcriptome.3) Train the user community in the use of arrays.Public-sector access to microarrays is critical to ensure that the large public investment in rice structural genomics is converted into public scientific and economic benefits. We will deliver a publicly available oligonucleotide microarray that will be useful for analysis of virtually any trait in rice, a repository for rice microarray expression data, and enhanced functional annotation of the rice genome. DeliverablesIn years one and two, 15000-element arrays will be distributed. In year three, 40,000-element arrays will be distributed. Information about the arrays and where to order them will be available on the project web site at http://www.tigr.org.

水稻因其基因组尺寸小、可用的基因组序列和易于转化而成为禾本科植物和谷物的模型。水稻基因的结构和功能分析对于玉米和小麦等其他经济上重要的谷物具有广泛的实际意义。一种方法是微阵列分析，生物学家可以在单个实验中测量数千个基因的表达水平。可以通过计算从这些数据中提取具有生物学意义的信息，然后用于为基因分配功能，并识别遗传调控网络。在这个项目中，我们将：1) 设计 40,000 个 70 聚体寡聚物并构建微阵列以供项目使用和公共分发。 2）构建项目数据的数据分析系统、数据库和用户界面。开发基于网络的水稻转录组数据挖掘工具。3) 对用户社区进行阵列使用培训。公共部门获取微阵列对于确保水稻结构基因组学的大量公共投资转化为公共科学和经济效益至关重要。 我们将提供一个公开可用的寡核苷酸微阵列，该阵列可用于分析水稻的几乎所有性状、水稻微阵列表达数据的存储库以及水稻基因组的增强功能注释。可交付成果 在第一年和第二年，将分发 15000 个元素的数组。第三年，将分发 40,000 个元素的数组。 有关阵列以及订购地点的信息将在项目网站 http://www.tigr.org 上提供。