A Rice Oligonucleotide Array

水稻因其基因组小、基因组序列可用、易于转化而成为禾本科和谷类的模式作物。水稻基因的结构和功能分析对玉米、小麦等其他重要经济作物具有广泛的实际意义。一种方法，微阵列分析，允许生物学家在一个实验中测量数千个基因的表达水平。通过计算可以从这些数据中提取具有生物学意义的信息，然后用于分配基因的功能，并识别基因调控网络。在本计画中，我们将：1）设计40，000个70-mer寡聚物并建构微阵列以供计画使用及公开发行。2)为项目数据构建数据分析系统、数据库和用户界面。开发基于网络的水稻转录组数据挖掘工具。3）培训用户社区使用微阵列公共部门获得微阵列对于确保水稻结构基因组学的大量公共投资转化为公共科学和经济效益至关重要。 我们将提供一个公开可用的寡核苷酸微阵列，这将是有用的分析几乎任何性状的水稻，水稻微阵列表达数据库，并增强功能注释的水稻基因组。在第一年和第二年，将分发15000个元素的阵列。第三年，四万阵的阵法，全部发放。 有关阵列的信息以及在哪里订购它们将在项目网站http://www.tigr.org上提供。

Rice has become a model for grasses and cereal because of its small genome size, available genome sequence, and ease of transformation. The structural and functional analysis of rice genes has broad practical implications for the other economically important cereals such as corn and wheat. One approach, microarray analysis, permits biologists to measure the expression levels of thousands of genes in single experiment. Biologically significant information can be extracted from these data computationally, and then used to assign functions to genes, and to identify genetic regulatory networks. In this project, we will:1) Design 40,000 70-mer oligomers and construct microarrays for project use and public distribution. 2) Construct a data analysis system, database and user interface for project data. Develop web-based tools for data mining the rice transcriptome.3) Train the user community in the use of arrays.Public-sector access to microarrays is critical to ensure that the large public investment in rice structural genomics is converted into public scientific and economic benefits. We will deliver a publicly available oligonucleotide microarray that will be useful for analysis of virtually any trait in rice, a repository for rice microarray expression data, and enhanced functional annotation of the rice genome. DeliverablesIn years one and two, 15000-element arrays will be distributed. In year three, 40,000-element arrays will be distributed. Information about the arrays and where to order them will be available on the project web site at http://www.tigr.org.