A Rice Oligonucleotide Array

水稻已经成为禾本科和谷类作物的典范，因为它的小基因组大小，可用的基因组序列，以及易于转化。水稻基因的结构和功能分析对其他具有重要经济意义的谷物如玉米和小麦具有广泛的实际意义。一种方法，微阵列分析，允许生物学家在一次实验中测量数千个基因的表达水平。生物学上重要的信息可以通过计算从这些数据中提取出来，然后用于将功能分配给基因，并确定遗传调控网络。在这个项目中，我们将：1)设计40000个70-mer低聚物并构建微阵列用于项目使用和公共分发。2)构建项目数据分析系统、数据库和用户界面。开发基于网络的水稻转录组数据挖掘工具。3)训练用户社区使用数组。公共部门使用微阵列对于确保对水稻结构基因组学的大量公共投资转化为公共科学和经济效益至关重要。我们将提供一个公开可用的寡核苷酸微阵列，它将用于分析水稻的几乎任何性状，一个水稻微阵列表达数据库，并增强水稻基因组的功能注释。在第一年和第二年，将分发15000个元素的数组。在第三年，将分配40,000个元素的数组。有关阵列的信息和订购地点将在项目网站http://www.tigr.org上提供。

Rice has become a model for grasses and cereal because of its small genome size, available genome sequence, and ease of transformation. The structural and functional analysis of rice genes has broad practical implications for the other economically important cereals such as corn and wheat. One approach, microarray analysis, permits biologists to measure the expression levels of thousands of genes in single experiment. Biologically significant information can be extracted from these data computationally, and then used to assign functions to genes, and to identify genetic regulatory networks. In this project, we will:1) Design 40,000 70-mer oligomers and construct microarrays for project use and public distribution. 2) Construct a data analysis system, database and user interface for project data. Develop web-based tools for data mining the rice transcriptome.3) Train the user community in the use of arrays.Public-sector access to microarrays is critical to ensure that the large public investment in rice structural genomics is converted into public scientific and economic benefits. We will deliver a publicly available oligonucleotide microarray that will be useful for analysis of virtually any trait in rice, a repository for rice microarray expression data, and enhanced functional annotation of the rice genome. DeliverablesIn years one and two, 15000-element arrays will be distributed. In year three, 40,000-element arrays will be distributed. Information about the arrays and where to order them will be available on the project web site at http://www.tigr.org.