A Rice Oligonucleotide Array

水稻因其基因组大小小、可获得的基因组序列和易于转化而成为牧草和谷物的典范。水稻基因的结构和功能分析对玉米和小麦等其他重要的经济谷物具有广泛的实际意义。一种名为微阵列分析的方法允许生物学家在一次实验中测量数千个基因的表达水平。生物上有意义的信息可以通过计算从这些数据中提取出来，然后用来为基因分配功能，并识别基因调控网络。在这个项目中，我们将：1)设计40,000个70-聚低聚物，并构建用于项目使用和公开分发的微阵列。2)构建项目数据分析系统、数据库和用户界面。3)培训用户群体使用阵列。公共部门获得微阵列对于确保在水稻结构基因组学上的大量公共投资转化为公共科学和经济利益至关重要。我们将提供一个公开可用的寡核苷酸微阵列，它将用于分析水稻的几乎任何性状，一个水稻微阵列表达数据的储存库，以及增强的水稻基因组功能注释。可交付在第一年和第二年内，将分发15000个元素的阵列。在第三年，将分布40,000个元素的阵列。有关阵列以及订购位置的信息将在项目网站上提供，网址为http://www.tigr.org.

Rice has become a model for grasses and cereal because of its small genome size, available genome sequence, and ease of transformation. The structural and functional analysis of rice genes has broad practical implications for the other economically important cereals such as corn and wheat. One approach, microarray analysis, permits biologists to measure the expression levels of thousands of genes in single experiment. Biologically significant information can be extracted from these data computationally, and then used to assign functions to genes, and to identify genetic regulatory networks. In this project, we will:1) Design 40,000 70-mer oligomers and construct microarrays for project use and public distribution. 2) Construct a data analysis system, database and user interface for project data. Develop web-based tools for data mining the rice transcriptome.3) Train the user community in the use of arrays.Public-sector access to microarrays is critical to ensure that the large public investment in rice structural genomics is converted into public scientific and economic benefits. We will deliver a publicly available oligonucleotide microarray that will be useful for analysis of virtually any trait in rice, a repository for rice microarray expression data, and enhanced functional annotation of the rice genome. DeliverablesIn years one and two, 15000-element arrays will be distributed. In year three, 40,000-element arrays will be distributed. Information about the arrays and where to order them will be available on the project web site at http://www.tigr.org.