Gene expression of colonic mucosa in patients with ulcerative colitis using oligonucleotide array-based technology

使用基于寡核苷酸阵列的技术研究溃疡性结肠炎患者结肠粘膜的基因表达

• 批准号: 13671349
• 负责人: MASUDA Hideki
• 金额: $ 2.11万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671349/
• 关键词: Gene chip; Osteopontin; gene expression; Ulcerative colitis; Inflammatory bowel diseases; Colon Cancer

Genome sequencing projects and the development of DNA array techniques have recently provided new tools that render a more comprehensive picture of the gene expression underlying the disease's state. We used oligonucleotide array-based technologies for the analysis of colon cancer. In addition, we report the analysis of mucosal gene expression in UC. Ten colonic tissue samples (7 UC and 3 control samples) were subjected to high-density oligonucleotide array analysis. 25 genes had a 3.0〜23.4-fold higher mRNA expression in UC samples compared with normal samples. Two genes showing more than a10-fold increase expression in UC samples were a macrophage metalloelastase and a osteopontin. It has been said that osteopontin important role in the pathogenesis of systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In conclusion, our present study indicates the possibility that osteopontin plays an important role in the pathogenesis of UC via increased immune activity.

基因组测序项目和DNA阵列技术的发展最近提供了新的工具，使一个更全面的图片的基因表达的疾病的状态。我们使用基于寡核苷酸阵列的技术来分析结肠癌。此外，我们报告了UC粘膜基因表达的分析。对10份结肠组织样品（7份UC和3份对照样品）进行高密度寡核苷酸阵列分析。25个基因在UC中的mRNA表达是正常对照组的3.0 ~ 23.4倍。两个基因在UC样本中表达增加超过10倍，分别是巨噬细胞金属弹性蛋白酶和骨桥蛋白。骨桥蛋白在系统性红斑狼疮（SLE）和类风湿性关节炎（RA）的发病机制中具有重要作用。总之，我们目前的研究表明，骨桥蛋白通过增加免疫活性在UC的发病机制中发挥重要作用的可能性。

项目成果

Yasuo Takahashi: "Up-regulation of Vitamin D3 up-regulated protein/gene in response to 5-fluorouracil in colon carcinoma SW620"Oncology Reports. 9・1. 75-79 (2002)

Yasuo Takahashi：“结肠癌 SW620 中维生素 D3 上调蛋白质/基因的响应”，《肿瘤学报告》9·1。

Hideki Masuda: "Distinct Gene Expression of Osteopontin in Patients with Ulcerative Colitis"Journal of Surgical Research. 107(in press). (2003)

Hideki Masuda：“溃疡性结肠炎患者骨桥蛋白的独特基因表达”外科研究杂志。

Yasuo Takahashi: "Clinical Application of Oligonucleotide prove Array for full-length gene sequencing of TP53 in Colon Cancer"Oncology. 64・1. 54-60 (2003)

Yasuo Takahashi：“寡核苷酸证明阵列在结肠癌中的全长基因测序的临床应用”肿瘤学 64・1（2003）。

Yasuo Takahashi: "Clinical application of Oligonucleotide probe array for full-length Gene sequencing of TP53 in colon cancer"Oncology. 64. 54-60 (2003)

Yasuo Takahashi：“寡核苷酸探针阵列对结肠癌 TP53 全长基因测序的临床应用”肿瘤学。

Hideki Masuda: "Distinct Gene Expression of Osteopontin in Patients with Ulceratire Colitis"Journal of Surgical Research. 107(in press). (2003)

Hideki Masuda：“溃疡性结肠炎患者骨桥蛋白的独特基因表达”外科研究杂志。