Regulation of plant metabolism in response to low oxygen concentrations

在这个建议中，我们的目标是表征信号成分，参与植物的积极响应，以适度降低氧浓度，因为它发生在生理条件下，在他们的组织。要做到这一点，我们将主要集中在调查信号通路，导致重新编程的基因表达，以响应低氧。在过去的几年中，大量的缺氧诱导的代谢和分子反应已经被我们表征。现在，我们想研究参与调节各种反应的传感和信号级联。我们将使用RT-PCR转录因子谱来鉴定参与调节基因表达和适应低氧的氧相关转录因子。将在体内和体外表征所选择的转录因子，以揭示它们的生理功能和它们的遗传相互作用以触发氧敏感启动子的转录。将在通过低氧响应的QTL分析鉴定的染色体切片中探索靶序列。荧光素酶报告筛选用于发现将基因表达变化与低氧联系起来的基因中的氧信号突变体。

In this proposal we aim to characterize signalling components that are involved in the proactive response of plants to a moderate decrease in the oxygen concentration as it occurs under physiological conditions within their tissues. To do this, we will mainly focus on investigating signalling pathways that lead to reprogramming of gene expression in response to low oxygen. In the past years, a vast array of hypoxia induced metabolic and molecular responses are already characterised by us. Now, we want to investigate the sensing and signalling cascade that is involved in regulating the various responses. We will use RT-PCR transcription factor profiling to identify oxygen related transcription factors that are involved in regulating gene expression and adaptation to low oxygen. The selected transcription factors will be characterised in-vivo and in-vitro to reveal their physiological function and their genetic interaction to trigger transcription of oxygen sensitive promoters. Target sequences will be explored in chromosomal sections that have been identified by QTL analysis of the low-oxygen response. A luciferase reporter screen is used to find oxygen signalling mutants defective in genes that link the changes in gene expression to low oxygen.