SBIR Phase I: Five-Dimensional Fluorescence Microscopy

这个小企业创新研究（SBIR）第一阶段项目建议开发一种创新的荧光显微镜方法，该方法将提供高空间分辨率和改进的对比度。正如以前的工作所证明的那样，所提出的荧光显微镜是独一无二的，因为它可以同时测量多个波长的寿命衰减曲线。荧光显微镜同时增加的光谱和时间分辨率将增强其效用，并允许设计更复杂的荧光实验，用于细胞和亚细胞系统的结构和功能成像。在这个项目中开发的产品的商业应用将作为一种仪器，对生物和医学许多领域的研究人员有用。另外的医疗应用，如内窥镜探针，用于识别组织类型和异常组织基于荧光光谱和寿命特性的差异，也设想。

This Small Business Innovation Research (SBIR)Phase I project proposes to develop an innovative approach to fluorescence microscopy that will provide high spatial resolution and improved contrast. As demonstrated from previous work, the proposed fluorescence microscope is unique because it provides simultaneous measurement of lifetime decay profiles at multiple wavelengths. The addition of simultaneous spectral and temporal resolution to the fluorescence microscope will enhance their utility and allow the design of more sophisticated fluorescence experiments for structural and functional imaging of cellular and sub-cellular systems. The commercial application of the product to be developed in this project will be as an instrumentation useful to researchers across many areas of biological and medical sciences. Additional medical applications such as endoscopic probes for the identification of tissue types and abnormal tissue based on differences in fluorescence spectral and lifetime properties are also envisioned.