SBIR Phase I: Characterization of the Metabolic Competency of Centrifugal Bioreactors

这个小型企业创新研究(SBIR)第一阶段项目建议检测用于培养悬浮哺乳动物细胞的新型离心式生物反应器(CBR)的生化和代谢特性。CBR实现了动物细胞在沸腾床中密度大于108个细胞/毫升的悬浮，其中营养液流动力抵消了离心力。第一阶段的总体目标是提供对CBR文化过程的坚实的科学理解。具体的技术目标包括测量CBR中具有代表性的细胞系的生长速度，根据细胞的营养消耗和代谢物生产建立CBR的稳态运行，并通过改变CBR入口处的溶解氧浓度来操纵稳态。该项目将在动物细胞培养生物反应器领域进行商业应用。拟议技术的主要影响将是降低商业动物细胞培养设备的规模和资本成本，并潜在地提高分泌蛋白产品的质量和一致性。

This Small Business Innovation Research (SBIR) Phase I project proposes to examine the biochemical and metabolic characteristics of a novel Centrifugal Bioreactor (CBR) used to culture suspension mammalian cells. The CBR achieves suspension of animal cells at densities greater than 10 8 cells/mL in a fluidized bed where nutrient liquid flow forces counteract centrifugal forces. The phase I overall goal is to provide a solid scientific understanding of the CBR culture process. The specific technical objectives include measuring the growth rate of a representative cell line in the CBR , establishing steady-state operation of the CBR in terms of nutrient consumption and metabolite production by the cells and manipulating the steady state by changing the dissolved oxygen concentration at the CBR inlet.The commercial application of this project will be in the area of animal cell culture bioreactors. The principal impact of the proposed technology will be to reduce the scale and capital costs of commercial animal cell culture equipment and to potentially improve the quality and consistency of secreted protein product.