SBIR Phase I: Characterization of the Metabolic Competency of Centrifugal Bioreactors

SBIR 第一阶段：离心生物反应器代谢能力的表征

• 批准号: 0319253
• 负责人: Heath Herman
• 金额: $ 9.97万
• 依托单位: Kinetic Biosystems, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0319253&HistoricalAwards=false
• 关键词: SBIR; Phase; Characterization; Metabolic; Competency

This Small Business Innovation Research (SBIR) Phase I project proposes to examine the biochemical and metabolic characteristics of a novel Centrifugal Bioreactor (CBR) used to culture suspension mammalian cells. The CBR achieves suspension of animal cells at densities greater than 10 8 cells/mL in a fluidized bed where nutrient liquid flow forces counteract centrifugal forces. The phase I overall goal is to provide a solid scientific understanding of the CBR culture process. The specific technical objectives include measuring the growth rate of a representative cell line in the CBR , establishing steady-state operation of the CBR in terms of nutrient consumption and metabolite production by the cells and manipulating the steady state by changing the dissolved oxygen concentration at the CBR inlet.The commercial application of this project will be in the area of animal cell culture bioreactors. The principal impact of the proposed technology will be to reduce the scale and capital costs of commercial animal cell culture equipment and to potentially improve the quality and consistency of secreted protein product.

这个小企业创新研究（SBIR）第一阶段项目提出了一种新的离心生物反应器（CBR）用于培养悬浮哺乳动物细胞的生化和代谢特征。CBR在流化床中实现了动物细胞的悬浮，其密度大于10 8个细胞/mL，其中营养液流动力抵消离心力。第一阶段的总体目标是提供一个坚实的CBR文化过程的科学理解。具体的技术目标包括测量CBR中代表性细胞系的生长速率，根据细胞的营养消耗和代谢产物产生建立CBR的稳态操作，并通过改变CBR入口处的溶解氧浓度来操纵稳态。所提出的技术的主要影响将是降低商业动物细胞培养设备的规模和资本成本，并可能提高分泌蛋白质产品的质量和一致性。