SBIR Phase II: New devices Bioaerosol Sampler for Accurate, Time-Resolved Characterization of Viable Microbes and their Genomes

SBIR 第二阶段：新设备生物气溶胶采样器，用于准确、时间分辨地表征活微生物及其基因组

• 批准号: 1853240
• 负责人: Patricia Keady
• 金额: $ 74.87万
• 依托单位: Aerosol Devices Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1853240&HistoricalAwards=false
• 关键词: SBIR; Phase; II; New; devices

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is to provide general commercial access to a new generation of affordable, high-efficiency aerosol samplers that will primarily be used in the Industrial Hygiene and Indoor Air Quality market. The collection technology in these new instruments is unique in that it captures, concentrates and preserves airborne microbes in the same physical state they exist as they are suspended in the air we breathe- a tremendous breakthrough for forensic aerosol analysis. This work optimizes a novel collection method that chronologically resolves air samples into a portable compact platform, which ensures purity, minimizes handling and is safe for mail. The sample output is delivered in small, sterile medical grade disposable plastics that are compatible with a broad range of users' analytical needs whether it be the military, health care, atmospheric researchers or indoor air quality sector. This instrumentation is portable, and requires no filters or chemical additions; it rapidly condenses airborne microbes out of ambient air by manipulating humidity, offering a reliable way to assess microbiological air pollution-indoors or out. This SBIR Phase II project proposes to optimize the design of condensation growth-based bioaerosol samplers for commercial validation, rapid manufacture and high-quality reproduction. The accurate assessment of airborne biological agents remains a tremendous scientific and practical challenge. The intellectual merit of this work lies in finally overcoming the technical barriers posed by conventional air sampling equipment, which require extensive sampling time and significantly compromises the very information military, medical and building science professionals need: what is the identity, distribution and abundance of airborne microbes. This team will use the latest forensic genetic sequencing technology to isolate the detection limits of this new collection equipment for common airborne pathogens and allergens. The objective is to validate these new filterless aerosol recovery instruments in controlled laboratory experiments, with a broad range of common pathogenic bioaerosols. The team will demonstrate how the sample preservation benefits of this technology, can be realized for commercial benefit in monitoring high-density indoor environments, including health care settings and public schools. Operating this new equipment in occupied indoor spaces, we anticipate collecting bioaerosol in excess of forensic detection limits in less than 30 minutes, while maintaining exceptional sample fidelity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该小企业创新研究 (SBIR) 项目的更广泛影响/商业潜力是为新一代经济实惠、高效的气溶胶采样器提供一般商业机会，这些采样器将主要用于工业卫生和室内空气质量市场。 这些新仪器中的收集技术的独特之处在于，它可以捕获、浓缩和保存空气中的微生物，使其保持与悬浮在我们呼吸的空气中相同的物理状态，这是法医气溶胶分析的巨大突破。 这项工作优化了一种新颖的收集方法，该方法可按时间顺序将空气样本解析到便携式紧凑平台中，从而确保纯度，最大限度地减少处理并且对邮件来说是安全的。 样品输出采用小型无菌医用级一次性塑料制成，可满足广泛用户的分析需求，无论是军事、医疗保健、大气研究人员还是室内空气质量部门。 该仪器是便携式的，不需要过滤器或化学添加物；它通过控制湿度快速凝结周围空气中的微生物，为评估室内或室外微生物空气污染提供了可靠的方法。 该 SBIR 第二阶段项目建议优化基于冷凝生长的生物气溶胶采样器的设计，以实现商业验证、快速制造和高质量再现。 准确评估空气中的生物制剂仍然是一个巨大的科学和实践挑战。 这项工作的智力价值在于最终克服了传统空气采样设备所带来的技术障碍，这些设备需要大量的采样时间，并且严重损害了军事、医学和建筑科学专业人员所需的信息：空气中微生物的身份、分布和丰度。 该团队将利用最新的法医基因测序技术来隔离这种新型采集设备对常见空气传播病原体和过敏原的检测限。 目的是在受控实验室实验中使用各种常见致病性生物气溶胶验证这些新型无过滤气溶胶回收仪器。 该团队将展示该技术的样本保存优势如何在监测高密度室内环境（包括医疗保健机构和公立学校）方面实现商业利益。 在占用的室内空间中操作这种新设备，我们预计在 30 分钟内收集超过法医检测极限的生物气溶胶，同时保持卓越的样本保真度。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Airborne murine coronavirus response to low levels of hypochlorous acid, hydrogen peroxide and glycol vapors

• DOI: 10.1080/02786826.2022.2120794
• 发表时间: 2022-09-12
• 期刊: AEROSOL SCIENCE AND TECHNOLOGY
• 影响因子: 5.2
• 作者: Gomez, Odessa;McCabe, Kevin M.;Hernandez, Mark
• 通讯作者: Hernandez, Mark