SBIR Phase II: New devices Bioaerosol Sampler for Accurate, Time-Resolved Characterization of Viable Microbes and their Genomes

SBIR 第二阶段：新设备生物气溶胶采样器，用于准确、时间分辨地表征活微生物及其基因组

• 批准号: 1853240
• 负责人: Patricia Keady
• 金额: $ 74.87万
• 依托单位: Aerosol Devices Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1853240&HistoricalAwards=false
• 关键词: SBIR; Phase; II; New; devices

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is to provide general commercial access to a new generation of affordable, high-efficiency aerosol samplers that will primarily be used in the Industrial Hygiene and Indoor Air Quality market. The collection technology in these new instruments is unique in that it captures, concentrates and preserves airborne microbes in the same physical state they exist as they are suspended in the air we breathe- a tremendous breakthrough for forensic aerosol analysis. This work optimizes a novel collection method that chronologically resolves air samples into a portable compact platform, which ensures purity, minimizes handling and is safe for mail. The sample output is delivered in small, sterile medical grade disposable plastics that are compatible with a broad range of users' analytical needs whether it be the military, health care, atmospheric researchers or indoor air quality sector. This instrumentation is portable, and requires no filters or chemical additions; it rapidly condenses airborne microbes out of ambient air by manipulating humidity, offering a reliable way to assess microbiological air pollution-indoors or out. This SBIR Phase II project proposes to optimize the design of condensation growth-based bioaerosol samplers for commercial validation, rapid manufacture and high-quality reproduction. The accurate assessment of airborne biological agents remains a tremendous scientific and practical challenge. The intellectual merit of this work lies in finally overcoming the technical barriers posed by conventional air sampling equipment, which require extensive sampling time and significantly compromises the very information military, medical and building science professionals need: what is the identity, distribution and abundance of airborne microbes. This team will use the latest forensic genetic sequencing technology to isolate the detection limits of this new collection equipment for common airborne pathogens and allergens. The objective is to validate these new filterless aerosol recovery instruments in controlled laboratory experiments, with a broad range of common pathogenic bioaerosols. The team will demonstrate how the sample preservation benefits of this technology, can be realized for commercial benefit in monitoring high-density indoor environments, including health care settings and public schools. Operating this new equipment in occupied indoor spaces, we anticipate collecting bioaerosol in excess of forensic detection limits in less than 30 minutes, while maintaining exceptional sample fidelity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）项目的更广泛的影响/商业潜力是为新一代负担得起的高效气溶胶采样器提供一般商业访问，该采样器将主要用于工业卫生和室内空气质量市场。 这些新仪器中的收集技术是独一无二的，因为它可以捕获，浓缩物和保留它们存在的物理状态，因为它们被悬挂在空中中，我们呼吸 - 这是法医气溶胶分析的巨大突破。 这项工作优化了一种新颖的收集方法，该方法按时间顺序将空气样品解析到便携式紧凑型平台中，从而确保纯度，最大程度地减少操作并安全邮件。 样品输出以小型的无菌医学级一次性塑料交付，与多种用户的分析需求兼容，无论是军事，医疗保健，大气研究人员还是室内空气质量部门。 该仪器是便携式的，不需要过滤器或化学添加；通过操纵湿度，它可以迅速从环境空气中凝结空气中的微生物，并提供一种评估微生物空气污染室内或外部的可靠方法。 该SBIR II期项目提议优化基于凝结生长生长的生物溶质采样器的设计，以进行商业验证，快速生产和高质量的繁殖。 对机载生物剂的准确评估仍然是一个巨大的科学和实用挑战。 这项工作的智力优点在于最终克服了传统的空气采样设备所带来的技术障碍，这些设备需要大量的抽样时间，并显着损害了军事，医学和建筑科学专业人员的非常损害：空中捕虫的身份，分布和丰富性是什么。 该团队将使用最新的法医遗传测序技术来隔离该新收集设备的检测限制，用于常见的空中病原体和过敏原。 目的是在受控实验室实验中验证这些无过滤器的气溶胶恢复仪器，并具有广泛的常见致病生物溶质剂。 该团队将展示该技术的样本保存益处如何实现，以在监视高密度的室内环境（包括医疗保健环境和公立学校）时获得商业益处。 我们预计在不到30分钟内收集超过法医检测限制的生物美感剂，同时保持出色的样本保真度。这项奖项反映了NSF的法定任务，并被认为是值得通过评估基金会的知识分子和更广泛的影响审查Criteria，在不到30分钟的时间内收集生物剂，这将在不到30分钟的时间内收集生物美感，并在不到30分钟的时间内收集生物剂。

项目成果

Airborne murine coronavirus response to low levels of hypochlorous acid, hydrogen peroxide and glycol vapors

• DOI: 10.1080/02786826.2022.2120794
• 发表时间: 2022-09-12
• 期刊: AEROSOL SCIENCE AND TECHNOLOGY
• 影响因子: 5.2
• 作者: Gomez, Odessa;McCabe, Kevin M.;Hernandez, Mark
• 通讯作者: Hernandez, Mark