SBIR Phase I: A Portable Electrochemical Based Pathogen Biosensor

SBIR 第一阶段：基于便携式电化学的病原体生物传感器

• 批准号: 0319676
• 负责人: Season Wong
• 金额: $ 10万
• 依托单位: Lynntech, Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0319676&HistoricalAwards=false
• 关键词: SBIR; Phase; Portable; Electrochemical; Based

This Small Business Innovation Research (SBIR) Phase I aims to develop an electrochemical DNA biosensor to simultaneously detect and identify multiple nucleic acid determinants of a variety of biological pathogens using a combination of dynamic hybridization, microfluidics, and electrochemical detection. The sensitivity of the detection method is high enough that polymerase chain reaction (PCR) amplification could be eliminated. The detection system would consist of a sampling device, sample lysis and hybridization chamber fluidically connected to an electrochemical cell for detection. With this method, specific target nucleic acid species captured by oligonucleotide probes on magnetic particles are released into neighboring working electrode where the nucleic acid targets are directly detected by electrochemical oxidation. This provides a method of rapid, highly sensitive, and specific DNA detection without the need for complicated sample purification procedures, exogenous labels, labile reagents, or expensive, heavy, power-hungry instrumentation. The commercial use of this product will be in military applications and homeland defense for prompt detection of potential bioterrorist attacks. Development of such devices for DNA and RNA detection without the need for amplification steps would also find wide application in the detection of pathogens in medical diagnostics, point-of-care clinical testing, environmental monitoring, agriculture, food production, and various industrial settings.

这个小企业创新研究（SBIR）第一阶段的目的是开发一种电化学DNA生物传感器，使用动态杂交，微流体和电化学检测的组合，同时检测和识别多种生物病原体的多个核酸决定簇。 检测方法的灵敏度足够高，可以消除聚合酶链反应（PCR）扩增。 检测系统将由采样装置、样品裂解和杂交室组成，所述样品裂解和杂交室流体连接到用于检测的电化学电池。 利用这种方法，由磁性颗粒上的寡核苷酸探针捕获的特定靶核酸物质被释放到相邻的工作电极中，在该工作电极中，通过电化学氧化直接检测核酸靶。 这提供了一种快速、高灵敏度和特异性DNA检测的方法，而不需要复杂的样品纯化程序、外源标记、不稳定的试剂或昂贵、笨重、耗电的仪器。 该产品的商业用途将用于军事应用和国土防御，以迅速检测潜在的生物恐怖袭击。在不需要扩增步骤的情况下开发用于DNA和RNA检测的此类装置还将广泛应用于医学诊断、护理点临床测试、环境监测、农业、食品生产和各种工业环境中的病原体检测。