SBIR Phase I: Development of a portable, sensitive, user-friendly electrochemical biosensor for detecting Pesticide residues

SBIR 第一阶段：开发便携式、灵敏、用户友好的电化学生物传感器，用于检测农药残留

• 批准号: 1940054
• 负责人: Lance Ford
• 金额: $ 22.5万
• 依托单位: Attogene Corp
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1940054&HistoricalAwards=false
• 关键词: SBIR; Phase; Development; portable; sensitive

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is to develop a portable, faster, cheaper, more sensitive, more consistent user-friendly electrochemical biosensor for detection of organophosphate and carbamate pesticide (OPaC) residues. While OPaC chemicals have greatly improved crop yields, they have also (1) caused accumulation of pesticide residues in the food chain, (2) promoted the generation of pesticide-resistant insects, and (3) contaminated the air, water and soil. OPaC residues, therefore, are a significant public health concern. The proposed sensor will: increase the sensitivity and specificity for monitoring OPaCs (2) reduce costs associated with pesticide monitoring, and (3) improve portability of monitoring devices. Finally, the proposed biosensor technology could be adaptable for analyzing biofluids, making it transformative in monitoring environmental exposures.This Small Business Innovation Research Phase I project proposes to develop a more sensitive, field-deployable electrochemical biosensor for the detection of organophosphate and carbamate pesticide (OPaC) residues. The system’s principle is that OPaCs bind/inhibit the activity of a highly sensitive designer acetylcholinesterase (AChE) enzyme, diminishing a readily detectable electrochemical current. At the system’s core is a novel AChE enzyme more sensitive to OPaC residues than those currently in use. The approach will further optimize the performance of this novel enzyme by rationally designing variants that decrease aggregation and increase/improve thermal stability, core packing, surface polarity, and backbone rigidity. Finally, the approach will enhance OPaC detection sensitivity even further by increasing the surface area of the electrochemical sensory apparatus. Briefly, the procedure is to submerge the sensor into a buffer to acquire and assign baseline data, add test samples to allow any OPaCs to bind to the optimized AChE enzyme on the sensor, add the AChE substrate acetylthiocholine to the sample, and measure electrochemical inhibition. Taken together, this novel biosensor will result in a major shift in the way OPaC analysis is performed and pave the way for reliable, sensitive, and low-cost field analysis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个小企业创新研究（SBIR）项目的更广泛的影响/商业潜力是开发一种便携式，更快，更便宜，更灵敏，更一致的用户友好的电化学生物传感器，用于检测有机磷和氨基甲酸酯农药（OPaC）残留。 虽然OPaC化学品大大提高了作物产量，但它们也（1）导致农药残留在食物链中积累，（2）促进产生抗药性昆虫，（3）污染空气、水和土壤。 因此，OPaC残留是一个重大的公共卫生问题。 申报的传感器将：提高监测OPaCs的灵敏度和特异性，（2）降低与农药监测相关的成本，以及（3）提高监测设备的便携性。最后，提出的生物传感器技术可以适用于分析生物流体，使其在监测环境暴露的变革。这个小企业创新研究第一阶段项目建议开发一种更灵敏，现场部署的电化学生物传感器，用于检测有机磷和氨基甲酸酯农药（OPaC）残留。该系统的原理是OPaCs结合/抑制高度敏感的乙酰胆碱酯酶（AChE）酶的活性，减少容易检测的电化学电流。 该系统的核心是一种新的AChE酶，它对OPaC残基比目前使用的那些更敏感。 该方法将通过合理设计减少聚集和增加/改善热稳定性、核心填充、表面极性和骨架刚性的变体来进一步优化这种新型酶的性能。 最后，该方法将通过增加电化学传感装置的表面积来进一步增强OPaC检测灵敏度。 简而言之，该程序是将传感器浸入缓冲液中以获取和分配基线数据，添加测试样本以允许任何OPaCs与传感器上优化的AChE酶结合，将AChE底物乙酰硫代胆碱添加到样本中，并测量电化学抑制。 总之，这种新型生物传感器将导致OPaC分析方式的重大转变，并为可靠、灵敏和低成本的现场分析铺平道路。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。