MRI/RUI: Acquisition of an Automated DNA Sequencing, Microsatellite, AFLP Analysis System for Integrated Research and Education in Population Biology at a HSI/PUI

MRI/RUI：获取自动 DNA 测序、微卫星、AFLP 分析系统，用于 HSI/PUI 的人口生物学综合研究和教育

• 批准号: 0321209
• 负责人: Anthony Metcalf
• 金额: $ 8.29万
• 依托单位: University Enterprises Corporation at CSUSB
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321209&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Automated; DNA

A grant has been awarded to California State University San Bernardino under the direction of Dr. Anthony Metcalf. This grant enables the acquisition of an automated DNA sequencing system for scientific research and education at a predominantly undergraduate, Hispanic serving university (HIS/PUI). The initial and primary uses of the instrumentation will facilitate the integration of education and research into population and evolutionary biology. The equipment lowers costs, greatly increases research efficiency, stimulates new research projects and collaborations, and allows the completion of projects that require DNA sequence or genotype analysis. The region served by this institution is equal or larger than nine of the States in the U.S. Yet, this critical technological aspect of research and training had not been previously met.With this instrument, the full arc of research and training in DNA sequencing, and genotyping will be available to faculty, staff, and most important, undergraduate and Master level student researchers. Current research and student training that immediately benefit from the award focus on regional geographic patterns of molecular genetic variation across a suite of taxa. These projects include conservation applications with a genetic analysis of a recent range expansion of the federally endangered Stephens' kangaroo rat, and the conservation genetics of a local restricted plant, the endangered Lane Mountain milk vetch. Three other student research projects that immediately benefit from the award focus on the relationships between habitat structure and gene flow across different taxa. These projects are:1) the role of watershed in affecting genetic differentiation in the California treefrog; 2) the isolating effects of recent uplift on geographically isolated populations of the zebra tailed lizard; and 3) the population genetics of the pygmy nuthatch, a sedentary bird that inhabits yellow pine forests on disjunct mountain tops in southern California. Another set of student projects examines the population structure of southern California populations of the mosquito Culex quinquefaciatus in relation to insecticide resistance, using hypervariable regions of the genome.A broader impact of this project is that automated DNA sequencing and genotype instrumentation will greatly enhance current research efforts into the nature of genetic variation in inland southern California taxa. This region has a very high level of endemism, and coupled with explosive human population growth, and the alteration of habitat, results in a concentration of threatened and endangered species in a limited geographic area. This primary research focus at this HIS/PUI institution will have broad application to the regional habitat planning and conservation efforts currently taking place. The research projects will allow the CSUSB faculty and community users to more fully integrate research and education. And, most importantly, it will allow the students to fully experience research-from the inception of a question, through the experimental and technical work, to the presentation and publication of their projects.

在安东尼·梅特卡夫博士的指导下，加州州立大学圣贝纳迪诺获得了一笔赠款。该补助金使人们能够在一所以本科生为主的西班牙裔大学（HIS/PUI）购买用于科学研究和教育的自动DNA测序系统。 仪器的初步和主要用途将促进教育和研究纳入人口和进化生物学。该设备降低了成本，大大提高了研究效率，刺激了新的研究项目和合作，并允许完成需要DNA序列或基因型分析的项目。该机构所服务的区域等于或大于美国的九个州。然而，这一研究和培训的关键技术方面以前没有得到过满足。有了这台仪器，教职员工，最重要的是，本科生和硕士生研究人员将可以获得DNA测序和基因分型的全部研究和培训。目前的研究和学生培训将立即受益于该奖项，重点关注一系列分类群中分子遗传变异的区域地理模式。这些项目包括保护应用，对联邦濒危的斯蒂芬斯袋鼠鼠最近的范围扩大进行遗传分析，以及对当地限制植物濒危的莱恩山紫云英进行遗传学保护。其他三个学生研究项目，立即受益于该奖项集中在栖息地结构和基因流之间的关系，在不同的类群。这些项目是：1）分水岭在影响加州树蛙遗传分化方面的作用; 2）最近的隆起对地理上孤立的斑尾蜥蜴种群的隔离影响;以及3）侏儒的种群遗传学，这是一种栖息在加州南部间断山顶上的黄松林中的栖息鸟类。另一组学生项目利用基因组的高变区研究了南加州蚊虫种群的种群结构与杀虫剂抗性的关系。该项目的更广泛的影响是，自动DNA测序和基因型仪器将大大加强目前对南加州内陆类群遗传变异性质的研究。这一地区的特有性非常高，再加上人口爆炸性增长和栖息地的改变，导致受威胁和濒危物种集中在有限的地理区域。这个HIS/PUI机构的主要研究重点将广泛应用于目前正在进行的区域栖息地规划和保护工作。这些研究项目将使CSUSB的教师和社区用户能够更充分地整合研究和教育。而且，最重要的是，它将允许学生充分体验研究-从一个问题的开始，通过实验和技术工作，到他们的项目的演示和出版。