MRI/RUI: Acquisition of Genetic Analyzer and Sequence Detection System for the Sonoma State University Core DNA Analysis Facility

MRI/RUI：为索诺玛州立大学核心 DNA 分析设施购置遗传分析仪和序列检测系统

• 批准号: 0321367
• 负责人: Derek Girman
• 金额: --
• 依托单位: Sonoma State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321367&HistoricalAwards=false
• 关键词: MRI; RUI; Acquisition; Genetic; Analyzer

This project, upgrading capabilities of a Core DNA Analysis Facility, builds on a successful NSF CCLI award. The acquisition of a capillary based genetic analyzer and a real-time PCR detection system is proposed to complete investigations in a variety of fields ranging from studies of gene regulation, to the mentoring and modeling of disease spread, to molecular systematics, and conservation genetics. Ten of the on-going projects use DNA sequencing for various purposes, including molecular systematics, mapping of phenotypic characters, examination of population structure, understanding biogeography, and identification and characterization of specific genes. Of four genotyping projects, three use analysis of highly variable microsatellites for fine scale population analyses, analyses of kinship (genetic fingerprinting), or mutation detection. One genotyping project uses AFLP analysis to conduct genome-wide sampling for use in GIS modeling that includes a genotypic "landscape." Two projects rely simply on genotype detection or mutation detection by analysis of the presence/absence or condition of PCR products. In line with the mission to foster combined research/education, this work carries a good success story.The project, accommodating the increased demand for usage of the facility by students and faculty, not only enhances faculty research programs, but also involves research training in an undergraduate institution. Moreover, teacher training is expected to impact on K-12 education.

这个项目，升级核心DNA分析设备的能力，建立在一个成功的NSF CCLI奖。本文拟引进毛细管遗传分析仪和实时PCR检测系统，以完成从基因调控研究、疾病传播指导和建模、分子系统学和保守遗传学等多个领域的研究。10个正在进行的项目将DNA测序用于各种目的，包括分子系统学、表型特征制图、种群结构检查、生物地理学理解以及特定基因的鉴定和表征。在四个基因分型项目中，有三个使用高度可变的微卫星进行精细群体分析、亲缘关系分析（遗传指纹）或突变检测。一个基因分型项目使用AFLP分析进行全基因组采样，用于包括基因型“景观”的GIS建模。两个项目仅仅依靠基因型检测或突变检测，通过分析PCR产物的存在/缺失或条件。与促进研究与教育结合的使命相一致，这项工作取得了很好的成功。该项目适应了学生和教师对设施使用需求的增加，不仅加强了教师的研究项目，而且还涉及了本科院校的研究培训。此外，教师培训预计将对K-12教育产生影响。