MRI/RUI: Acquisition of Genetic Analyzer and Sequence Detection System for the Sonoma State University Core DNA Analysis Facility

这个项目，升级核心DNA分析设施的能力，建立在一个成功的NSF CCLI奖。收购毛细管为基础的遗传分析仪和实时PCR检测系统的建议，以完成各种领域的调查，从基因调控的研究，指导和建模的疾病传播，分子系统学，和保护遗传学。正在进行的项目中有10个利用DNA测序达到各种目的，包括分子系统学、绘制表型特征图、检查种群结构、了解植物地理学以及鉴定和描述特定基因。在四个基因分型项目中，有三个使用高度可变的微卫星分析进行精细规模的人口分析，亲属关系分析（遗传指纹）或突变检测。一个基因分型项目使用AFLP分析进行全基因组取样，用于GIS建模，包括基因型“景观”。“两个项目仅仅依靠基因型检测或突变检测，通过分析PCR产物的存在/不存在或条件。该项目符合促进研究/教育相结合的使命，具有很好的成功经验。该项目适应了学生和教师对使用该设施的日益增长的需求，不仅加强了教师的研究计划，而且还涉及本科院校的研究培训。此外，教师培训预计将影响K-12教育。

This project, upgrading capabilities of a Core DNA Analysis Facility, builds on a successful NSF CCLI award. The acquisition of a capillary based genetic analyzer and a real-time PCR detection system is proposed to complete investigations in a variety of fields ranging from studies of gene regulation, to the mentoring and modeling of disease spread, to molecular systematics, and conservation genetics. Ten of the on-going projects use DNA sequencing for various purposes, including molecular systematics, mapping of phenotypic characters, examination of population structure, understanding biogeography, and identification and characterization of specific genes. Of four genotyping projects, three use analysis of highly variable microsatellites for fine scale population analyses, analyses of kinship (genetic fingerprinting), or mutation detection. One genotyping project uses AFLP analysis to conduct genome-wide sampling for use in GIS modeling that includes a genotypic "landscape." Two projects rely simply on genotype detection or mutation detection by analysis of the presence/absence or condition of PCR products. In line with the mission to foster combined research/education, this work carries a good success story.The project, accommodating the increased demand for usage of the facility by students and faculty, not only enhances faculty research programs, but also involves research training in an undergraduate institution. Moreover, teacher training is expected to impact on K-12 education.