Apple Functional Genomics

苹果功能基因组学

• 批准号: 0321701
• 负责人: Schuyler Korban
• 金额: $ 165.97万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0321701&HistoricalAwards=false
• 关键词: Apple; Functional; Genomics

AbstractThe overall goal of this NSF project is to establish structural tools for apple genomics.The apple, Malus x domestica Borkh., is the most important deciduous tree fruit cropgrown in the United States. This project will focus on developing structural genomicresearch tools with emphasis on flowering, fruiting, and pathogen response in apple.The first phase of this project is to construct an expressed sequence tag (EST) databaseof 120,000 sequences from flowering and fruiting tissues, and from tissues responding topathogen infection, specifically to the bacterial pathogen Erwinia amylovora (the causalorganism of fire blight disease) and to the fungal pathogen Venturia inaequalis (thecausal organism of apple scab disease). These will then be used to generate a set of30,000 unigene clones that will be subjected to 3' sequencing. An apple genome dataset will begenerated and integrated into a national Rosaceae Genome Database. Aneducational and outreach component of this project will involve developing web-basedmodules for use in educating high school teachers and students on the conceptsstructural genomics.

美国国家科学基金会（NSF）这个项目的总体目标是建立苹果基因组学的结构工具。是美国种植的最重要的落叶果树。本项目的重点是开发苹果开花、结果和病原菌反应的结构基因组学研究工具，第一阶段是建立一个包含12万个苹果开花、结果组织和病原菌反应组织序列的表达序列标签（EST）数据库，特别是针对细菌病原体梨火疫病菌（Erwiniamylovora）（火疫病的病原体）和真菌病原体苹果黑星病菌（Venturia inaequalis）（苹果黑星病的病原体）。然后这些将被用来产生一组30，000个单基因克隆，这些克隆将被进行3'测序。苹果基因组数据集将被生成并整合到国家蔷薇科基因组数据库中。该项目的教育和推广部分将涉及开发基于网络的模块，用于对高中教师和学生进行结构基因组学概念的教育。