Biophotonics: Cross-Reactive Suspension Arrays of Sensors

我们正在构建编码珠上的交叉反应疏水传感器悬浮阵列来模拟嗅觉系统。交叉反应悬液阵列将在流式细胞术中提供尿液中所有疏水分子的瞬时指纹。在资助期结束时，一个典型的阵列将由6到64个选定的分子传感器组成，每个传感器最多有5000个读数（珠）。该阵列将在收集的尿液样本上进行验证，用于代谢物筛选。疏水指纹将与疾病状态相关。该方法将首先用于库欣病、肾上腺腺瘤、肾上腺癌、多毛症和先天性肾上腺增生。这种方法将扩展到其他分析物和其他样品。

0321972StojanovicA suspension array of cross-reacting hydrophobic sensors on encoded beads is being constructed to model the olfactory system. The cross-reactive suspension array will provide in flow cytometry an instantaneous fingerprint of all hydrophobic molecules present in urine. A typical array at the end of funding period will consist of six to 64 selected molecular sensors with up to 5000 readings (beads) per sensor. The arrays will be validated on urine specimens collected for metabolite screening. The hydrophobic fingerprints will be correlated with disease states. This approach will be initially demonstrated on Cushing's disease, adrenal adenoma, adrenal carcinoma, hirsutism and congenital adrenal hyperplasia. This approach will be expanded to other analytes and other samples.