Molecular mapping of QTL and candidate genes governing phytosterol content in winter oilseed rape (Brassica napus) and Indian mustard (Brassica juncea)

控制冬季油菜（Brassica napus）和印度芥菜（Brassica juncea）植物甾醇含量的QTL和候选基因的分子图谱

• 批准号: 163823895
• 负责人: Dr. Christian Möllers
• 金额: --
• 依托单位: Department of Genetics
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/163823895?language=en
• 关键词: Molecular; mapping; QTL; candidate; genes

Plant sterols, also called phytosterols, comprise a group of plant steroidal compounds. They are of interest because they play a crucial role in plant growth and development and as part of the human diet they are known for their blood cholesterol-lowering effects. High phytosterol contents occur in seeds of oilseed crops, the relevance of which remains hitherto unknown. In contrast to Arabidopsis nothing is know about the genes governing phytosterol content in Brassica species. In previous work, a large genetic. variation for phytosterol content has been found among German winter oilseed rape (Brassica napus) cultivars. So far no information is available on total phytosterol contents or individual components in Indian mustard B. juncea. Suitable segregating doubled haploid populations of B. napus, and of B. juncea have already been developed and will be used in the present project to map QTL for individual and total seed phytosterol content as well as for other seed quality traits. Key candidate genes of the phytosterol biosynthetic pathway with known sequences from Arabidopsis will be sequenced from the progenitor species Brassica rapa, Brassica nigra and Brassica oleracea to develop locus specific PCR primers. Those locus specific primers will be used to sequence the pertinent alleles in the respective amphidiploid species B. napus and B. juncea. Allelic sequence differences between the parents of the doubled haploid populations will be used to map the candidate genes. Results will reveal if positions of candidate genes overlap with those of QTL for phytosterol content and if genetic variation in phytosterol content is related to variation in other seed quality traits.

植物甾醇，又称植物甾醇，由一组植物甾体化合物组成。它们之所以令人感兴趣，是因为它们在植物的生长和发育中发挥着关键作用，作为人类饮食的一部分，它们以降低血液胆固醇的效果而闻名。植物甾醇在油料作物种子中含量很高，其相关性迄今尚不清楚。与拟南芥不同的是，人们对控制油菜中植物甾醇含量的基因一无所知。在以前的工作中，有一个很大的基因。德国冬季油菜(Brassica Napus)品种间植物甾醇含量存在差异。到目前为止，还没有关于印度芥菜中植物甾醇总含量或个别成分的信息。甘蓝型油菜和芥菜型油菜双单倍体群体的适宜分离已被开发出来，并将在本项目中用于定位个体和总种子植物甾醇含量以及其他种子品质性状的QTL。利用拟南芥中已知序列的植物甾醇生物合成途径的关键候选基因，将从油菜、黑甘蓝和甘蓝的祖先物种中进行测序，以开发基因座特异的聚合酶链式反应引物。这些座位特异的引物将被用来对各自的双二倍体物种甘蓝型油菜和芥菜型油菜的相关等位基因进行测序。将利用加倍单倍体群体亲本之间的等位基因序列差异来定位候选基因。结果将揭示候选基因的位置是否与植物甾醇含量的QTL重叠，以及植物甾醇含量的遗传变异是否与其他种子品质性状的变异有关。