Transfer of thalamocortical and its control by GABAergic mechanisms in the adolescent mouse cerebral cortex in vitro

青春期小鼠大脑皮层丘脑皮质的转移及其 GABA 能机制的控制体外

• 批准号: 163904573
• 负责人: Professor Dr. Heiko J. Luhmann
• 金额: --
• 依托单位: Instituto de Fisiología Celular
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/163904573?language=en
• 关键词: Transfer; thalamocortical; its; control; GABAergic

Sensory information is encoded in distributed neuronal assemblies in cortical and subcortical areas. To investigate the role of GABAergic interneurons in the formation and propagation of such neuronal assemblies we will use confocal Ca2+ imaging, whole-cell patch clamp and high-densitiy multi electrode array (HD-MEA) recordings to analyse spatiotemporal patterns of coactive neurons after stimulation of the ventrobasal thalamus in an in vitro slice preparation of the mouse barrel cortex. Part of the experiments will be performed in animals expressing GFP under the control of the GAD67 promotor to enable identification of GABAergic interneurons. Confocal Ca2+ imaging will be used to characterize the spatial domain of stimulus-induced neuronal assemblies and their dependence on stimulus features. Targeted patch-clamp recordings from neurons characterized by their contribution to stimulus-induced assemblies and/or their neurotransmitter phenotype will be used to identify the synaptic processes during assembly formation and to selectively add/remove GABAergic interneurons from neuronal assemblies. In addition, pharmacological interference with GABAergic neurotransmission will be used to analyze how inhibition contributes to assembly formation and propagation. HD-MEA recordings will deliver the spike pattern occurring after application of a specific stimulus, allowing the analysis of the dynamic processes leading to the formation of neuronal assemblies. The results of these experiments will contribute to our understanding how sensory information is encoded and processed in nervous systems.

感觉信息被编码在分布在皮层和皮层下区域的神经元集合中。为了研究GABA能中间神经元在这种神经元组装体的形成和增殖中的作用，我们将使用共聚焦Ca 2+成像、全细胞膜片钳和高密度多电极阵列（HD-MEA）记录来分析刺激小鼠桶状皮层的腹侧基底丘脑后共同活动神经元的时空模式。部分实验将在GAD 67启动子控制下表达GFP的动物中进行，以鉴定GABA能中间神经元。共焦Ca 2+成像将用于表征刺激诱导的神经元组装的空间域及其对刺激特征的依赖性。来自神经元的靶向膜片钳记录（其特征在于其对刺激诱导的组装和/或其神经递质表型的贡献）将用于鉴定组装形成期间的突触过程，并选择性地从神经元组装中添加/去除GABA能中间神经元。此外，GABA能神经传递的药理学干扰将用于分析抑制如何有助于组装形成和传播。HD-MEA记录将提供在施加特定刺激后发生的尖峰模式，允许分析导致神经元组装形成的动态过程。这些实验的结果将有助于我们理解感觉信息是如何在神经系统中编码和处理的。