ADVANCE Fellows Award - NMR Microscopy of Structure-Function Relationships and Microfluidics in Biofilms and Cellular suspensions

高级研究员奖 - 生物膜和细胞悬浮液中结构功能关系和微流体的核磁共振显微镜

• 批准号: 0340709
• 负责人: Sarah Codd
• 金额: $ 38.75万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0340709&HistoricalAwards=false
• 关键词: ADVANCE; Fellows; Award; NMR; Microscopy

This ADVANCE Fellows award is to support the academic career development of the awardee in engineering. The overall mission of the ADVANCE Program is to increase the participation of women in the scientific and engineering workforce through the increased representation and advancement of women in academic science and engineering careers. It is anticipated that the ADVANCE award will not only advance the PI's career as an engineering researcher, but also allow her to serve as a role model and mentor to women students who are pursuing careers in engineering, or related fields. This research will extend NMR microscopy to study the structure-function and micro-fluidics surrounding growth and decay of biofilms in capillaries and porous media and investigate cell transport through microchannels. Biofilms are microbial colonies that grow on surfaces. While much is known about the growth state of free bacteria, little is known about the structure-function relationships when cells are in the biofilm state and it is known that biofilms play a significant role in the resistance of bacteria to antibiotic treatment. The use of novel NMR microscopy contrast agents that are "turned on" with function, as well as the use of iron-tagged cells will allow some of the vital questions surrounding biofilm function to be answered. It is expected that this research, which is relevant to numerous micro-fluidic applications such as biosensors and "lab on a chip" systems, will have significant broader impacts in the analysis of the transport of viruses and microbes in filtration separations. NMR microscopy methods will allow noninvasive measurement of time and length scale dependent displacements within opaque systems such as cell suspensions and porous media and will provide unique data for testing of conceptual and numerical models of transport in these systems. The collaboration between chemical and bioengineering, with physics and chemistry, will enhance the breadth of understanding of micro-fluidics. The awardee intends to advance diversity in her department by encouraging women graduate and undergraduate students to work with her on this highly relevant biofilm research. This ADVANCE Fellows award will be managed by two divisions, BES and CTS.

该预先研究员奖是为了支持该工程获奖者的学术职业发展。预先计划的总体使命是通过增加妇女在学术科学和工程职业中的代表性和进步来增加妇女在科学和工程劳动力中的参与。 可以预料，预先奖励不仅会推进PI作为工程研究人员的职业，而且还可以使她成为从事工程或相关领域从事职业的女学生的榜样和导师。这项研究将扩展NMR显微镜，以研究毛细血管和多孔培养基生物膜生长和衰减的结构功能和微氟化物，并通过微通道研究细胞的传输。 生物膜是在表面上生长的微生物菌落。尽管对游离细菌的生长状态知之甚少，但对于细胞处于生物膜状态时的结构功能关系知之甚少，众所周知，生物膜在细菌对抗生素治疗的抗性中起着重要作用。使用功能“打开”的新型NMR显微镜对比剂以及使用铁的细胞的使用将允许回答围绕生物膜功能的一些重要问题。 可以预期，这项研究与众多微富集应用有关，例如生物传感器和“芯片上的实验室”系统，将在过滤分离中对病毒和微生物的运输分析产生更大的影响。 NMR显微镜方法将允许在不透明系统（例如细胞悬浮液和多孔介质）内无创测量时间和长度尺度依赖性位移，并将提供独特的数据，用于测试这些系统中概念和数值传输模型。化学与生物工程之间与物理和化学之间的合作将增强对微富集学的了解。获奖者打算通过鼓励女性研究生和本科生与她合作进行这项高度相关的生物膜研究，以提高其部门的多样性。 该预先研究员奖将由BES和CTS的两个部门管理。