A Student-Accessible Model for Human Genetics Using Fast Plants and Microsatellite Markers

使用快速植物和微卫星标记的学生可访问的人类遗传学模型

• 批准号: 0340910
• 负责人: Douglas Wendell
• 金额: $ 7.49万
• 依托单位: Oakland University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0340910&HistoricalAwards=false
• 关键词: Student; Accessible; Model; Human; Genetics

An experimental system that models human genetics is being developed for instructional laboratories. The experiments involve microsatellite markers in rapid cycling Brassica rapa (RCBr), or Fast Plants. The objectives of the project include development of microsatellite markers for RCBr and analysis methods that make them accessible to the instructional lab, strains of RCBr with defined microsatellite genotypes resembling human genetic variation, a pedigree analysis experiment for linkage analysis with microsatellite markers and the model genetic disease anthocyaninless, a virtual laboratory in which students will exchange data with peers, analyze data, and allow the instructor to track their data analysis, and a prototype web site and manual for high school teachers and students to analyze data generated by the college labs. The types of genetic analysis the students can use in these instructional labs include techniques of microsatellite analysis, assessment of informativeness of families, inheritance of highly polymorphic markers, and haplotype analysis. Since genetic linkage cannot be established from a single family, students use the virtual lab to pool data. In this way, students can perform experiments that accurately model human genetic analysis and gain experience with molecular markers. The web-based system facilitates analysis of complex data, pooling of individual student data, and instructor tracking of analysis.

一个模拟人类遗传学的实验系统正在为教学实验室开发。实验涉及快速循环油菜（RCBr）或快速植物的微卫星标记。该项目的目标包括：开发RCBr的微卫星标记和分析方法，使其可用于教学实验室；具有与人类遗传变异相似的确定的微卫星基因型的RCBr菌株；利用微卫星标记进行连锁分析的系谱分析实验；并允许讲师跟踪他们的数据分析，以及一个原型网站和手册，供高中教师和学生分析由大学实验室产生的数据。学生可以在这些教学实验室中使用的遗传分析类型包括微卫星分析技术、家庭信息评估、高度多态性标记的遗传和单倍型分析。由于不能从单个家庭建立遗传联系，学生们使用虚拟实验室来汇集数据。通过这种方式，学生可以进行精确模拟人类基因分析的实验，并获得分子标记的经验。基于网络的系统有助于分析复杂数据，汇集个人学生数据，并跟踪教师的分析。