Acquisition of a Nanoelectrospray Source and Supporting Equipment

在化学研究仪器和设施（CRIF）计划的支持下，伦斯勒理工学院化学系将购买纳米电喷雾（nano-ESI）源和配套设备。该设备将放置在RPI的质谱分析设施中，将加强在许多领域的研究，包括1)在色谱分离过程中确认蛋白质置换分子的功效；B)从头开始蛋白质设计和方法设计，以增强肽的质谱信号；C)低聚糖复杂混合物的分析；D)重组蛋白生产；e)阐明蛋白质在外植生物材料上的沉积。质谱（MS）是一种用于探测内部结构细节和获得大量有机、生物有机和有机金属分子的分子组成的技术。纳米esi是一种经过验证的方法，可使感兴趣的分子电离而不碎裂。因此，质谱和纳米esi的结合是发展最快和应用最广泛的分析仪器技术之一，用于较大的生物分子，否则会在电离时破碎。这些研究将在许多领域产生重大影响，尤其是生物化学。

With support from the Chemistry Research Instrumentation and Facilities (CRIF) Program, the Department of Chemistry at Rensselaer Polytechnic Institute will purchase a nanoelectrospray (nano-ESI) source and supporting equipment. This equipment, which will be placed in the Mass Spectrometry Facility at RPI, will enhance research in a number of areas including 1) confirmation of the efficacy of protein displacement molecules during chromatographic separations; b) de novo protein design and design of methodologies to enhance mass spectrometric signals from peptides; c) analysis of complex mixtures of oligosaccharides; d) recombination protein production; and e) elucidation of protein deposition on explanted biomaterials. Mass spectrometry (MS) is a technique used to probe intimate structural details and to obtain the molecular compositions of a vast array of organic, bioorganic, and organometallic molecules. Nano-ESI is a proven method for ionizing the molecule of interest without fragmentation. Thus the combination of MS and nano-ESI is one of the fastest growing and most widely used analytical instrumentation techniques for larger biomolecules that would otherwise fragment upon ionization. These studies will have a significant impact in a number of areas, especially biochemistry.