Microstructured fibres (MSFs) for nanoelectrospray ionization (nano-esi) and chromatographic applications

用于纳电喷雾电离 (nano-esi) 和色谱应用的微结构纤维 (MSF)

• 批准号: 239026-2011
• 负责人: Oleschuk, Richard
• 金额: $ 5.1万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514420
• 关键词: Microstructured; fibres; MSFs; nanoelectrospray; ionization

Our laboratory is interested in the development of novel separation and detection methods for chemical analysis. Chemical analysis forms the basis for discovery in a wide variety of disciplines (e.g. environment, medical and "omics" related research areas). The development of analysis systems that are faster, require less sample with increased sensitivity are in constant demand and become critical to probing e.g. biological and chemical systems at cellular/sub-cellular levels. We plan to adopt a novel optical fiber material for use as both a substrate for open tubular liquid chromatography and for high sensitivity nanoelectrospray ionization mass spectrometry. Fibres with a number of different hole sizes and hole patterns have only recently become commercially available. The multiple, parallel silica channels presented by the MSF act as a promising substrate for a nanoOTLC column, as they have diameters near the ideal range for interactions (1 to 3 µm). Furthermore, the application of MSF's for nanoelectrospray has the potential to substantially improve the way in which nanoelectrospray is performed. Conventional emitters are tapered and as a result suffer from continuous clogging and offer very poor emitter-emitter reproducibility. MSF's can function as an emitter by pumping liquid through the pores of the fibre and as a result of the numerous flow paths virtually eliminate problems associated with clogging. Furthermore, if each hole could act as an independent emitter then a significant increase in ion production and sensitivity would result. As such, a greater number of electrospray emitters increase the ionization efficiency leading to enhanced sensitivity. MSFs with different hole patterns and hole sizes will be utilized to manufacture nanoelectrospray emitters that both improve nanospray robustness and enhance nanoelectrospray sensitivity. The application of these novel fibres to both liquid chromatography and nanoelectrospray has the potential to completely revolutionize both research areas.

我们的实验室对开发用于化学分析的新型分离和检测方法感兴趣。化学分析构成了各种学科（例如环境，医学和“组学”相关研究领域）发现的基础。不断需要开发更快、需要更少样品且灵敏度增加的分析系统，并且对于在细胞/亚细胞水平上探测例如生物和化学系统变得至关重要。我们计划采用一种新型的光纤材料，既用作开管液相色谱的基质，又用于高灵敏度的纳米电喷雾电离质谱。具有许多不同孔尺寸和孔图案的纤维最近才商业上可获得。MSF提供的多个平行二氧化硅通道作为nanoOTLC柱的有前途的基底，因为它们的直径接近相互作用的理想范围（1至3 μm）。此外，MSF用于纳米电喷雾的应用具有实质上改进执行纳米电喷雾的方式的潜力。传统的发射器是锥形的，因此遭受连续堵塞，并提供非常差的发射器-发射器再现性。MSF可以通过将液体泵送通过纤维的孔隙来起到发射器的作用，并且由于众多的流动路径，实际上消除了与堵塞相关的问题。此外，如果每个孔可以充当独立的发射器，则将导致离子产生和灵敏度的显著增加。因此，更大数量的电喷雾发射器增加电离效率，导致灵敏度增强。具有不同孔图案和孔尺寸的MSF将用于制造纳米电喷雾发射器，其既改善纳米喷雾鲁棒性又增强纳米电喷雾灵敏度。这些新型纤维在液相色谱和纳米电喷雾中的应用有可能彻底改变这两个研究领域。