SGER: The Fate of Duplicated Genes in an Invasive Polyploid Species

同源异型基因，或那些通过多倍体事件复制的基因，可能以反映其复制的水平表达，或者其表达可能发生显著变化。本文提出的技术最近已被描述用于异源多倍体物种基因表达的分化。具体问题要解决，以评估theddynamics的WESTERN基因在异源多倍体入侵物种，大米草是：1。在不同拷贝的大米草中是否存在序列差异？maritima，S. alterniflora; S. anglica arose，S. xtownsendii和新发现的F1杂种S. x neyrautii？2.）在大米草、它的亲本种或二倍体祖先之间Wessel基因座的表达变化是否不同？3.）第三章大米草内Wyndrome是否存在差异变异？PCR、DNA克隆和测序以及cDNA单链构象多态性（SSCP）的结合将提供比以前使用的技术更精确的关于入侵异源多倍体物种的基因组织和功能的数据。将对RT-PCR产物进行直接测序，以确认所有结果。所进行的分析将提供深入了解thedynamics和随后的基因型和表型的变化，由于杂交之间的historicallopatric类群，homeopathy基因，异源多倍体化。

Homeologous genes, or those genes duplicated by polyploid events, may be expressed at levels reflective of their duplication, or their expression may be changed dramatically. The techniquesproposed here have been very recently described with the application toward the differentiationof gene expression in allopolyploid species. Specific questions to be addressed to assess thedynamics of the Waxy gene in the allopolyploid invasive species, Spartina anglica are: 1.) Arethere sequence differences between the different copies of Waxy in: Spartina anglica; its diploidprogenitors S. maritima, S. alterniflora; the sterile F1 hybrid from which S. anglica arose, S. xtownsendii; and the newly discovered F1 hybrid, S. x neyrautii? 2.) Does variation in expressionof the Waxy locus vary between Spartina anglica, its parental species or diploid ancestors? 3.) Isthere differential variation of Waxy within Spartina anglica? A combination of PCR, cloning andsequencing of DNA and single stranded conformation polymorphisms (SSCP) of cDNA willprovide more precision than previously used techniques to provide data on genic organization and function in an invasive allopolyploid species. Direct sequencing of RT-PCR products will beperformed for confirmation of all results. The analyses performed will provide insight into thedynamics and subsequent genotypic and phenotypic changes due to the hybridization betweenhistorically allopatric taxa, homeologous genes, and allopolyploidization.