The Function of HASTY in Arabidopsis

HASTY在拟南芥中的功能

• 批准号: 0346050
• 负责人: R. Poethig
• 金额: --
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0346050&HistoricalAwards=false
• 关键词: Function; HASTY; Arabidopsis

The transport of molecules across the nuclear membrane is primarily regulated by members of the importin beta family of proteins. Although the function of these proteins is well characterized in yeast and mammals, very little is known about their function in plants. The experiments described in this project are designed to reveal the function of HASTY, the Arabidopsis orthologue of Exportin5. Exportin5 has been implicated in the nuclear export of miRNAs and tRNAs in mammals, and the mutant phenotype of hasty suggests that it may have a similar function in plants. This hypothesis will be tested by examining the effect of hasty mutations on the sub-cellular distribution of miRNAs and tRNAs, and by identifying the molecules with which the HASTY protein physically interacts in vivo and in vitro. Screens for mutations that suppress the phenotype of hst-3 have been conducted in order to identify genes that are functionally related to HST. Positional cloning will be used to identify the gene affected by one such mutation, soh, and its function will be characterized. At present, HASTY is the only orthologue of Exportin5 in higher organisms for which mutant alleles exist. These mutations provide a unique opportunity to study the function of this important protein, and may provide new insights into the biogenesis and function of miRNAs as well.

分子穿过核膜的运输主要由蛋白质的importin β家族的成员调节。虽然这些蛋白质的功能在酵母和哺乳动物中得到了很好的表征，但对它们在植物中的功能知之甚少。本项目中描述的实验旨在揭示HASTY的功能，HASTY是拟南芥中Exportin 5的直向同源物。 Exportin 5与哺乳动物中miRNA和tRNA的核输出有关，hasty的突变表型表明它可能在植物中具有类似的功能。该假设将通过检查快速突变对miRNA和tRNA的亚细胞分布的影响，以及通过鉴定HASTY蛋白在体内和体外物理相互作用的分子来测试。已经进行了抑制hst-3表型的突变的筛选，以鉴定与HST功能相关的基因。定位克隆将被用于鉴定受这样一种突变影响的基因，soh，并将表征其功能。目前，HASTY是高等生物中存在突变等位基因的Exportin 5的唯一直向同源物。 这些突变为研究这种重要蛋白的功能提供了独特的机会，也可能为miRNAs的生物起源和功能提供新的见解。