An AP-MALDI-FTICR Mass Spectrometer for Proteomics Research

用于蛋白质组学研究的 AP-MALDI-FTICR 质谱仪

• 批准号: 0352451
• 负责人: James Bruce
• 金额: --
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0352451&HistoricalAwards=false
• 关键词: AP; MALDI; FTICR; Mass; Spectrometer

This award supports development of a novel high sensitivity, atmospheric pressure, matrix-assisted laser desorption/ionization (AP-MALDI) Fourier transform Ion Cyclotron Resonance mass spectrometer (FTICR-MS) to advance proteomics research capabilities. Specific areas of ion source development targeted in this proposal will include matrix effects and reactivity, and improved ion capture and transmission. Atmospheric pressure operation presents the opportunity to investigate the functional requirements of MALDI matrix materials and carefully characterize each component individually. The resultant development of a high sensitivity AP-MALDI ion source, combined with the accurate mass, tandem MS/MS capabilities of FTICR-MS will provide an ideal platform for proteomics research and protein identification. These efforts will result in an instrument that is capable of both APMALDI and ElectroSpray Injection, giving accurate mass measurements for optimal peptide mass fingerprinting and allowing coupling with on-line chromatography for analysis of protein mixture digests. The intellectual merit of the following proposal centers on three aspects 1) the development of improved sensitivity of AP-MALDI sources through implementation of multiple ionization sources and matrix optimization combined with advances in ion optics, 2) a new design for an FTICR instrument compatible with lower cost wide-bore NMR magnets, and 3) new methods to exploit accurate mass, tandem MS capabilities with APMALDI- FTICR-MS technology for proteomics research. Such an instrument, when coupled with automation and on-line chromatography will result in much higher throughput protein identification capabilities than is currently achieved. As planned, the instrument can make use of relatively inexpensive wide bore magnets currently used for NMR, leading to significantly lower cost FTICR-MS instruments. This will lead to increased training of graduate student researchers in the skills involved in FTICR-MS, increased dissemination and adaptation of next-generation proteomics research and increased collaboration with, and involvement of graduate student education in industry, national laboratory and academic research programs.

该奖项支持开发一种新型高灵敏度、大气压、基质辅助激光解吸/电离（AP-MALDI）傅立叶变换离子回旋共振质谱仪（FTICR-MS），以提高蛋白质组学研究能力。本提案针对的离子源开发的具体领域将包括基质效应和反应性，以及改进的离子捕获和传输。大气压操作为研究MALDI基质材料的功能要求和仔细表征每个组分提供了机会。由此产生的高灵敏度AP-MALDI离子源的发展，结合准确的质量，串联MS/MS的FTICR-MS的能力，将提供一个理想的平台，蛋白质组学研究和蛋白质鉴定。这些努力将产生一种能够进行APMALDI和电喷雾注射的仪器，为最佳肽质量指纹图谱提供准确的质量测量，并允许与在线色谱法耦合以分析蛋白质混合物。以下建议的智力价值集中在三个方面：1）通过实施多个电离源和矩阵优化结合离子光学的进步来开发AP-MALDI源的改进的灵敏度，2）与较低成本宽孔NMR磁体兼容的FTICR仪器的新设计，以及3）利用精确质量的新方法，串联质谱功能与APMALDI-FTICR-MS技术用于蛋白质组学研究。这样的仪器，当与自动化和在线色谱相结合时，将导致比目前实现的高得多的通量蛋白质鉴定能力。按照计划，该仪器可以利用目前用于NMR的相对便宜的宽孔磁体，从而显著降低FTICR-MS仪器的成本。这将导致研究生研究人员在FTICR-MS中所涉及的技能方面的培训增加，下一代蛋白质组学研究的传播和适应增加，以及研究生教育在工业，国家实验室和学术研究计划中的合作和参与增加。