ION-ELECTRON REACTIONS IN VIBRATIONALLY COOLED MALDI FTICR MS

振动冷却 MALDI FTICR MS 中的离子电子反应

• 批准号: 7602005
• 负责人: PETER B. O'CONNOR
• 金额: $ 0.97万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602005
• 关键词: American; Boston; Charge; Computer Retrieval of Information on Scientific Projects Database; Data; Dissociation; Electrons; Funding; Grant; Hydrogen; Institution; Ions; Journals; Mass Spectrum Analysis; Motion; Names; National Center for Research Resources; Paper; Parents; Pattern; Peer Review; Phosphorylation Site; Reaction; Research; Research Personnel; Resources; SHFM1 gene; Societies; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; Time; United States National Institutes of Health; Universities; Writing; instrument; interest; irradiation; medical schools; pressure; protein aminoacid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Because of the interesting new fragmentation patterns available with ECD and EDD, there's a strong interest in implementation of ion-electron reaction in the MALDI FTMS instrument. The Vibrationally Cooled (VC) MALDI built at the NCRR Mass Spectrometry Resource at Boston University School of Medicine is required for cooling of the vibrational motion of the ions and thus increases the abundance of multiply charged species in MALDI. SWIFT-isolated multiply charged ions were irradiated with low energy electrons and electron capture, hydrogen atom loss, and c,a fragment ions (all species that are expected for radical reactions) were detected. Efficiency of fragments is low due to the low abundance of the parent ion, but the fragment ion yield is rougly comparable to electrospray spectra. Thus, we have shown ECD of doubly charged MALDI ions. However, due to the much greater abundance, ion electron reactions were also attempted with the 1+ and 1- ion species. Irradiation of singly charged positive parent ions using electrons of 9-14 eV produced small peaks of further ionized species as well as extensive fragmentation of c,z,b,y,a ions. This new technique is thus named electron induced dissociation, and appears to be very promising for sequencing peptides in high pressure MALDI FTMS. Particularly the new technique will be useful for sequencing peptides with PTM¿s since the fragments have been noted to preserve labile groups such as phosphorylation sites. Electron capture dissociation and electron induced dissociation (EID) techniques were obtained for the first time for High pressure MALDI FTMS. These data were presented at the 52d American Society of Mass Spectrometry in Nashville, TN and are currently being written up for submission as a scientific paper in a peer-reviewed journal.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 由于ECD和EDD提供了有趣的新碎裂模式，因此在MALDI FTMS仪器中实现离子-电子反应引起了浓厚的兴趣。在波士顿大学医学院NCRR质谱库建造的振动冷却(VC)MALDI是冷却离子振动运动所必需的，从而增加了MALDI中多电荷物种的丰度。 用低能电子照射快速分离的多电荷离子，检测到电子俘获、氢原子损失和c，a碎片离子(所有可能发生自由基反应的物种)。由于母体离子丰度低，碎片的效率很低，但碎片离子的产率与电喷雾光谱大致相当。因此，我们展示了双电荷MALDI离子的ECD。然而，由于丰度大得多，也尝试了与1+和1-离子物种的离子电子反应。 用9-14 eV的电子辐照单电荷正离子母体离子会产生进一步电离的小峰以及c、z、b、y、a离子的广泛碎裂。这项新技术因此被称为电子诱导解离，在高压MALDI FTMS中对多肽进行测序似乎是非常有前途的。特别是，这项新技术将有助于用PTM？S对多肽进行测序，因为已经注意到这些片段保留了不稳定的基团，如磷酸化位点。 首次获得了高压MALDI FTMS的电子俘获解离和电子诱导解离(EID)技术。这些数据是在田纳西州纳什维尔举行的52d美国质谱学学会上公布的，目前正在撰写，作为一篇科学论文发表在同行评议的期刊上。