Studies of Antifreeze Proteins and Related Overwintering Adaptations in Arctic and Subarctic Insectes

北极和亚北极昆虫抗冻蛋白及相关越冬适应的研究

基本信息

  • 批准号:
    0352851
  • 负责人:
  • 金额:
    $ 18.5万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Standard Grant
  • 财政年份:
    2004
  • 资助国家:
    美国
  • 起止时间:
    2004-06-01 至 2006-05-31
  • 项目状态:
    已结题

项目摘要

This is a collaborative proposal by Principal Investigators at the University of Alaska-Fairbanks and Purdue University. Antifreeze proteins (AFPs) are found in a number of diverse organisms including fish, insects, spiders, mites, plant, and bacteria. While there is tremendous structural variation among these AFPs they all appear to lower the freezing point of water by a similar non-colligative mechanism. However, the insect AFPs are, by far, the most active, generally functioning to prevent freezing in freeze susceptible insects by inhibiting ice nucleating agents and innoculative freezing across the cuticle. However, a few AFP-producing insects are freeze tolerant and the function of AFPs in these is unknown. Two insect AFPs have been characterized. One type has been described from two species of beetles, but there is variation in sequence, etc., both within and between the species. Until recent research identified 18 species with AFPs, these proteins had not been reported in Alaskan or arctic insects. Certain of these have been chosen for further study, presenting the opportunity to extend understanding of insect AFPs to extremely cold tolerant species, and in the case of the beetle Cucujus clavipes ,to compare populations from arctic Alaska to those from Indiana. The mean winter supercooling point of Alaskan Cucujus larvae are -42oC, with some individuals supercooling to -57oC, while that of Indiana larvae is -24oC. AFP activity in Alaskan Cucujus is, by far, the highest ever described, even though the AFPs are similar to those of other beetles. Understanding the structure/function relationships of the Cucujus AFPs, and the enhancers (other proteins and polyols) which increase their activity, is of prime concern. In addition to AFPs, a suite of other overwintering adaptations (extreme dehydration to 1/3 normal body water, multimolar glycerol concentrations, diapause) contribute to the low supercooling points of Alaskan Cucujus. These are being studied, and using microarray technology and other means to monitor molecular markers, the Principal Investigators will attempt to understand the integration of these adaptations. Additional investigations involve the structure/function relationships of three other Alaskan beetle AFPs, and that of the insect type of AFP. Two of the beetle species are freeze tolerant and this is the first study of AFPs from freeze tolerant insects. The Principal Investigators will continue to screen additional species for the presence of AFPs (to identify interesting candidates for future study), and to monitor microhabitat temperatures and overwintering mortality of select species. The broader impact of this study concerns its effects on 1) biological education and 2) applications of AFPs in science and technology. Post-docs, PhD and undergraduate students, and high school teachers will work on this project. The Principal Investigators believe that the integrated training (in modern molecular techniques such as microarray, field biology, physiology of cold tolerance, protein biochemistry, etc.) at they will receive provides critical preparation for success in today's highlyintegrative and changing research environment. This will provide a direct benefit to teaching in biology. In addition to research papers, the Principal Investigators will incorporate these studies in review articles, symposium presentations. Also, as they are the most active AFPs known, insect AFPs (especially from Alaskan beetles) have tremendous potential for applied studies. For example, they have generated transgenic plants producing insect AFPs which lower the plant freezing temperature. Similar work using Alaskan beetle AFPs should be even more productive.
这是阿拉斯加大学费尔班克斯分校和普渡大学的主要研究人员的合作提案。 抗冻蛋白(AFP)存在于多种生物体中,包括鱼、昆虫、蜘蛛、螨、植物和细菌。虽然这些AFP之间存在巨大的结构差异,但它们似乎都通过类似的非依数性机制降低水的冰点。然而,到目前为止,昆虫AFP是最活跃的,通常通过抑制冰成核剂和跨角质层的接种冷冻来防止易冻昆虫中的冷冻。 然而,一些产生AFP的昆虫是耐冷冻的,并且AFP在这些昆虫中的功能尚不清楚。两种昆虫AFP已被表征。从两种甲虫中描述了一种类型,但在序列等方面存在变异,在物种内部和物种之间。 直到最近的研究发现了18种具有AFP的物种,这些蛋白质在阿拉斯加或北极昆虫中还没有报道。其中某些已被选择用于进一步研究,提供了将昆虫AFP的理解扩展到极耐冷物种的机会,并且在甲虫Cucujus clavipes的情况下,将来自阿拉斯加北极的种群与来自印第安纳州的种群进行比较。阿拉斯加库蚊幼虫的冬季过冷却点平均为-42 ℃,个别可达-57 ℃,而印第安纳州幼虫的冬季过冷却点平均为-24 ℃。到目前为止,阿拉斯加Cucujus中的AFP活性是有史以来最高的,尽管AFP与其他甲虫相似。理解Cucujus AFP的结构/功能关系以及增加其活性的增强剂(其他蛋白质和多元醇)是首要关注的问题。除了AFP,一套其他的越冬适应(极端脱水1/3正常的身体水分,多摩尔甘油浓度,滞育)有助于阿拉斯加库库的低过冷却点。这些正在研究中,并使用微阵列技术和其他手段来监测分子标记,主要研究者将试图了解这些适应的整合。其他调查涉及其他三个阿拉斯加甲虫AFP的结构/功能关系,以及昆虫类型的AFP。其中两种甲虫是耐寒的,这是首次从耐寒昆虫中提取AFP的研究。 主要研究人员将继续筛选其他物种是否存在AFP(以确定未来研究的感兴趣候选者),并监测微生境温度和选定物种的越冬死亡率。 这项研究的更广泛的影响涉及它对1)生物教育和2)科学和技术中的AFP应用的影响。 博士后,博士和本科生,以及高中教师将在这个项目上工作。 主要研究者认为,综合培训(现代分子技术,如微阵列,野外生物学,耐寒生理学,蛋白质生物化学等)在他们将收到提供了在今天的高度一体化和不断变化的研究环境中取得成功的关键准备。这将对生物学教学有直接的好处。除了研究论文,主要研究者将把这些研究纳入综述文章,研讨会演讲。 此外,由于它们是已知的最活跃的AFP,昆虫AFP(特别是来自阿拉斯加甲虫)具有巨大的应用研究潜力。例如,他们已经培育出了转基因植物,这些转基因植物能产生昆虫AFP,从而降低植物的冷冻温度。使用阿拉斯加甲虫AFP的类似工作应该更有成效。

项目成果

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John Duman其他文献

063 Functions of insect antifreeze proteins and glycolipids
  • DOI:
    10.1016/j.cryobiol.2013.09.069
  • 发表时间:
    2013-12-01
  • 期刊:
  • 影响因子:
  • 作者:
    John Duman;Henry Vu
  • 通讯作者:
    Henry Vu

John Duman的其他文献

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{{ truncateString('John Duman', 18)}}的其他基金

Collaborative Research: Novel Thermal Hysteresis Glycolipid Antifreeze in Insects and Plants
合作研究:昆虫和植物中的新型热滞糖脂防冻剂
  • 批准号:
    1025929
  • 财政年份:
    2010
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Standard Grant
Collaborative research: Deep Supercooling to -100C or Lower in Alaska Populations of the Beetle Cucujus clavipes
合作研究:将阿拉斯加的 Cucujus clavipes 甲虫种群深度过冷至 -100C 或更低
  • 批准号:
    0618342
  • 财政年份:
    2006
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Structure/Function Studies of Antifreeze Proteins and Their Enhancers from the Beetle Dendroides canadensis
加拿大树突甲虫抗冻蛋白及其增强剂的结构/功能研究
  • 批准号:
    0212907
  • 财政年份:
    2002
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Collaborative Research: Studies of Antifreeze Proteins in Arctic and Nearctic Insects
合作研究:北极和近北极昆虫抗冻蛋白的研究
  • 批准号:
    0004446
  • 财政年份:
    2001
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Structure/Function Studies of Antifreeze Protein From the Beetle Dendroides canadensis
加拿大树突甲虫抗冻蛋白的结构/功能研究
  • 批准号:
    9808376
  • 财政年份:
    1998
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Plant and Insect Thermal Hysteresis Antifreeze Proteins
植物和昆虫热滞抗冻蛋白
  • 批准号:
    9117903
  • 财政年份:
    1992
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Studies on Hemolymph Ice Nucleator Involved in Insect Cold Tolerance
血淋巴冰核参与昆虫耐寒性的研究
  • 批准号:
    8709872
  • 财政年份:
    1987
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Studies of Hemolymph Proteins Involvef in Insect Cold Tolerance
昆虫耐寒性涉及血淋巴蛋白的研究
  • 批准号:
    8416551
  • 财政年份:
    1985
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Standard Grant
Insect Ice Nuclear Proteins: Purification and Physical-Chemical Properties
昆虫冰核蛋白:纯化和理化性质
  • 批准号:
    8109708
  • 财政年份:
    1981
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Standard Grant
Role of Macromolecular Antifreeze in Insect Low Temperature Tolerance
高分子防冻剂在昆虫耐低温中的作用
  • 批准号:
    7703475
  • 财政年份:
    1977
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Standard Grant

相似海外基金

Structural studies on antifreeze proteins
抗冻蛋白的结构研究
  • 批准号:
    400823-2010
  • 财政年份:
    2010
  • 资助金额:
    $ 18.5万
  • 项目类别:
    University Undergraduate Student Research Awards
Structure and function studies on fish and wheat antifreeze proteins
鱼类和小麦抗冻蛋白的结构与功能研究
  • 批准号:
    385032-2009
  • 财政年份:
    2009
  • 资助金额:
    $ 18.5万
  • 项目类别:
    University Undergraduate Student Research Awards
Studies of Antifreeze Proteins and Related Overwintering Adaptations in Arctic and Anarctic Insects
北极和南极昆虫的抗冻蛋白及相关越冬适应的研究
  • 批准号:
    0352919
  • 财政年份:
    2004
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Standard Grant
Antifreeze Proteins in Antarctic Fishes: Integrated Studies of Freezing Environments and Organismal Freezing Avoidance, Protein Structure-Function and Mechanism, Genes & Evolut
南极鱼类的抗冻蛋白:冷冻环境与生物体避免冷冻的综合研究、蛋白质结构功能与机制、基因
  • 批准号:
    0231006
  • 财政年份:
    2003
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Structure/Function Studies of Antifreeze Proteins and Their Enhancers from the Beetle Dendroides canadensis
加拿大树突甲虫抗冻蛋白及其增强剂的结构/功能研究
  • 批准号:
    0212907
  • 财政年份:
    2002
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Collaborative Research: Studies of Antifreeze Proteins in Arctic and Nearctic Insects
合作研究:北极和近北极昆虫抗冻蛋白的研究
  • 批准号:
    0004446
  • 财政年份:
    2001
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
Collaborative Research: Studies of Antifreeze Proteins in Arctic and Nearctic Insects.
合作研究:北极和近北极昆虫抗冻蛋白的研究。
  • 批准号:
    0117104
  • 财政年份:
    2001
  • 资助金额:
    $ 18.5万
  • 项目类别:
    Continuing Grant
STRUCTURAL STUDIES ON ANTIFREEZE PROTEINS
抗冻蛋白的结构研究
  • 批准号:
    2750133
  • 财政年份:
    1997
  • 资助金额:
    $ 18.5万
  • 项目类别:
Structure-Function Studies of Antifreeze Proteins
抗冻蛋白的结构功能研究
  • 批准号:
    6946436
  • 财政年份:
    1997
  • 资助金额:
    $ 18.5万
  • 项目类别:
Structure-Function Studies of Antifreeze Proteins
抗冻蛋白的结构功能研究
  • 批准号:
    6542364
  • 财政年份:
    1997
  • 资助金额:
    $ 18.5万
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