A High Speed Confocal Microscope for Tracking Single Molecules

用于追踪单分子的高速共焦显微镜

基本信息

批准号：
0353030
负责人：
Jeffrey Urbach
金额：
$ 45.53万
依托单位：
Georgetown University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-01 至 2008-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0353030&HistoricalAwards=false
关键词：
Speed Confocal Microscope Tracking Single

项目摘要

This award provides support for the application of recent advances in high speed imaging technology to confocal microscopy. The goal of the effort is the development of a spinning disk confocal microscope with the combination of speed and sensitivity required to enable imaging and tracking of individual fluorescently labeled proteins rapidly diffusing in three dimensions. Optical imaging of subcellular processes is a critical tool in cell biology, but rapid dynamical processes involving small numbers of proteins are difficult or impossible to visualize using existing instruments. The development effort faces significant challenges for systems integration, image processing, and data handling. Performance goals include the acquisition of high resolution confocal images at up 1000 frames per second with fast z-scanning in order to allow for full 3-dimensional image stacks at high rates. Integrated software and hardware will be developed for imaging near the fundamental detection limits, and for handling and processing the prodigious amounts of data to be generated. The capabilities of the instrument will be tested by imaging previously unobservable dynamics in cytoskeletal networks, growth and development of axons, and other fundamental cell biological processes. As part of the effort, graduate students and postdoctoral fellows will be trained in relevant aspects of instrument development and software development.

该奖项为高速成像技术最新进展的应用提供了支持。努力的目的是开发旋转磁盘共聚焦显微镜与速度和灵敏度的结合，以启用成像和跟踪单个荧光标记的蛋白质在三个维度中迅速扩散。亚细胞过程的光学成像是细胞生物学的关键工具，但是涉及少量蛋白质的快速动力学过程很难或不可能使用现有仪器可视化。开发工作面临系统集成，图像处理和数据处理的重大挑战。性能目标包括以每秒1000帧的速度以快速的Z扫描方式获取高分辨率共聚焦图像，以便以高速率获得完整的3维图像堆栈。集成软件和硬件将开发用于在基本检测极限附近进行成像，并处理和处理要生成的大量数据。该仪器的能力将通过对先前无法观察到的细胞骨架网络，轴突的生长和发育以及其他基本细胞生物学过程中的动态进行成像进行测试。作为努力的一部分，研究生和博士后研究员将接受仪器开发和软件开发的相关方面的培训。