Live cell, high speed and resolution, spectral confocal microscope

描述（由申请人提供）：本申请来自马萨诸塞州伍兹霍尔的海洋生物实验室（MBL），要求提供购买高端共聚焦显微镜的资金。我们介绍了蔡司LSM-710 NLO/LSM Live DUO的案例。拟议的仪器将配备一系列的配件，根据我们的用户群的需求-特别是我们的主要用户。LSM-710将取代2002年购买的中央显微镜设施（CMF）中的蔡司LSM-510。新系统利用了激光器、探测器和软件组件的最新进展。例如，光谱成像的速度比LSM-510快得多。灵敏度和信噪比也有所提高。总之，710在许多方面都更高效。我们进一步提出，710具有显着的优势，竞争工具，并详细讨论这些。然而，认识到这是一个迅速变化的领域，如果获得这笔赠款，我们将重新评估情况是否发生重大变化。 我们提出的研究主要集中在活细胞成像和细胞动力学。提交的研究项目分为四组。第一部分包括NIH资助的住院研究和访问研究，包括我们的5个主要用户项目.其中四个主要项目得到了研究人员的支持，他们总共获得了9项相关的个人研究者NIH资助（1项优异奖和8项R01）。常驻研究包括来自伍兹霍尔联合会内两个研究所的申请;海洋生物研究所 实验室（MBL）和伍兹霍尔海洋研究所（WHOI）。两者都进行广泛的研究，具有很强的生物医学成分。第一类的访问研究包括MBL惠特曼研究人员和MBL细胞动力学计划的附属成员。首席调查员指导后者。我们在第一类中包括11个项目。我们的第二类用户包括由其他联邦机构资助的研究人员的应用程序（4个项目：NSF和ONR）。我们的第三类包括非联邦资助的访问调查员（2个项目）。最后，在第4类中，我们包括5个相关MBL课程的简短描述，在博士后到教授级别提供，这将利用新的工具。其中两项也得到了NIH赠款的支持。 蔡司710将被安置在CMF中，CMF在维护高端仪器，使其可供广大用户使用以及培训研究人员优化仪器使用方面具有典范性的历史。在MBL安装仪器的商业实体的支持由来已久，特别是卡尔蔡司公司的这一应用。 新设计的效率产生了几个环境优势，许多工作都与购买这种高端仪器密切相关。这些都进行了详细讨论。

DESCRIPTION (provided by applicant): This application, from the Marine Biological Laboratory (MBL), Woods Hole, MA, requests funding for the purchase of a high end confocal microscope. We present our case for the Zeiss LSM-710 NLO/LSM Live DUO. The proposed instrument will be equipped with an array of accessories dictated by the needs of our user group - in particular our major users. The LSM-710 will replace a Zeiss LSM-510 in the Central Microscopy Facility (CMF) that was purchased in 2002. The new system takes advantage of recent advancements in lasers, detectors and software components. Spectral imaging, for example, is accomplished at a much faster rate than the LSM-510. Sensitivity and signal-to-noise are also improved. The 710 is, in short, more efficient in many ways. We further propose that the 710 has significant advantages over competing instruments and discuss these in detail. However, recognizing that this is a rapidly changing field we would reappraise the situation for significant changes if awarded this grant. The majority of our proposed research focuses on live cell imaging and cell dynamics. The submitted research projects are subdivided into four groups. The first comprises NIH funded resident and visiting re- search and include our 5 major user projects. Four of these major projects are supported by researchers' holding a total of nine relevant individual investigator NIH grants (1 Merit Award and 8 R01s). Resident research includes applications from two of the institutes within the Woods Hole Consortium; the Marine Biological Laboratory (MBL) and the Woods Hole Oceanographic Institution (WHOI). Both undertake a broad range of re- search, with strong biomedical components. Visiting research in this first category covers MBL Whitman investigators and adjunct members of the MBL Cellular Dynamics Program. The Principal Investigator directs the latter. We include 11 projects in this first category. Our second user category includes applications from investigators funded by other federal agencies (4 projects: NSF and ONR). Our third category includes visiting investigators with non-federal funding (2 projects). Lastly, in category 4, we include short descriptions for 5 relevant MBL courses, offered at the post-doctoral to professorial level, which would make use of the new instrument. Two of these are also supported by NIH grants. The Zeiss 710 would be housed in the CMF, which has an exemplary history of maintaining high end instrumentation, making it available to a broad spectrum of users, and training researchers in the instrument's optimal use. There is a long history of support from commercial entities installing instrumentation at the MBL, notably for this application from Carl Zeiss Inc. The efficiency of the newer design yields several environmental advantages and a number of jobs are intimately connected with the purchase of this high end instrument. These are discussed in detail.