DISSERTATION RESEARCH: Effects of Larval Competition on Vector Competence of Container Mosquitoes Aedes Albopictus and Aedes Aegypti

论文研究：幼虫竞争对容器蚊子白纹伊蚊和埃及伊蚊媒介能力的影响

• 批准号: 0407689
• 负责人: L.Philip Lounibos
• 金额: $ 1.15万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0407689&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Effects; Larval; Competition

Effects of larval competition on vector competence of container mosquitoes Aedes albopictus and Aedes aegyptiProject Summary: . Competition due to limited food and high larval density occurs commonly among mosquitoes that occupy container habitats and influences their survivorship, growth, development, and adult size. Little is known, however, as to how competitive interactions among larvae influence the susceptibility of adult females to infection and transmission (i.e., vector competence) of mosquito-borne pathogens. This proposal tests whether variation in mosquito performance known to arise from larval competition is associated with variation in vector competence for dengue virus. A series of experiments varying larval density will test for effects of competition on vector competence of natural populations and laboratory colonies of Aedes aegypti and Aedes albopictus, the two most important transmitters of dengue virus in nature. Adult mosquitoes derived from competition experiments will be given a dengue-infectious blood meal, and vector competence will be measured with standard virological procedures. Progress to date using Sindbis, a model virus, showed that uncrowded larval conditions consistently resulted in superior mosquito performance compared to crowded conditions but, counterintuitively, resulted in decreased infection with Sindbis. The proposed experiments with dengue, an unrelated virus, will indicate the generality of results and whether mosquitoes from the laboratory and natural populations differ in responses. This research is novel for exploring how ecological factors in an early life stage may influence vector competence in adult, blood-feeding insects.

幼虫竞争对白纹伊蚊和埃及伊蚊媒介能力的影响由于食物有限和幼虫密度高，占据集装箱栖息地的蚊子之间经常发生竞争，影响它们的生存、生长、发育和成虫大小。然而，关于幼虫之间的竞争性相互作用如何影响成年雌性对蚊媒病原体的感染和传播(即媒介能力)的易感性，人们知之甚少。这项建议测试了已知由幼虫竞争引起的蚊子性能变化是否与登革热病毒媒介能力的变化有关。一系列改变幼虫密度的实验将测试竞争对自然种群和实验种群埃及伊蚊和白纹伊蚊媒介能力的影响，埃及伊蚊和白纹伊蚊是自然界中两个最重要的登革热病毒传播者。来自竞争实验的成年蚊子将被给予具有登革热感染的血粉，媒介能力将使用标准病毒学程序进行测量。迄今为止，使用模式病毒Sindbis的进展表明，与拥挤的条件相比，非拥挤的幼虫条件始终导致更好的蚊子性能，但与直觉相反的是，导致Sindbis的感染减少。拟议中的登革热病毒实验将表明结果的普遍性，以及实验室蚊子和自然种群的蚊子反应是否不同。这项研究对于探索早期生命阶段的生态因素如何影响成虫的媒介能力是新颖的。