Evolution of Reproductive Isolation in Cyprinodon

鲤鱼生殖隔离的进化

• 批准号: 0417338
• 负责人: Astrid Kodric-Brown
• 金额: --
• 依托单位: University of New Mexico
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0417338&HistoricalAwards=false
• 关键词: Evolution; Reproductive; Isolation; Cyprinodon

PROJECT SUMMARYEvolution of reproductive isolation in Cyprinodon Astrid Kodric-BrownUnderstanding processes that create and maintain species is a fundamental goal of modern biology. The proposed research will examine the roles of behavior, ecology, and offspring fitness in limiting hybridization between a native, endangered species, the Comanche Springs pupfish and an exotic species, the sheepshead minnow. The two species were geographically isolated, but have recently been brought into physical contact due to human activities. The proposed research seeks to identify the reproductive barriers that limit matings between individuals of the two species, and affect the viability and fertility of hybrid offspring. The strength of reproductive barriers will be determined by observing matings between Comanche Springs pupfish, sheepshead minnow, and their hybrids in artificial ponds. Preferences of Comanche Springs pupfish, sheepshead minnow and hybrid females will be tested by examining the responses of females to simultaneous presentations of visual and olfactory cues from each type of male. Male-male interactions will also be observed to determine species-specific differences in male mating behavior or competitive ability. The viability of hybrids will be determined by comparing the number of offspring produced by intraspecific and interspecific matings, then rearing the offspring under standardized conditions to compare growth rates, mortality, and sex ratio.The results of the proposed study will elucidate mechanisms that promote the divergence between lineages and contribute to understanding the resistance or susceptibility to hybridization in North American freshwater fishes. The project will contribute to training of graduate and undergraduate students and to informing the general public through posters and exhibits. Students will receive training in conceptual approaches as well as in experimental and field methods. The findings will be presented in posters and talks at regional and national meetings, and will be published both in professional journals and in lay publications.

项目总结鲤鱼生殖隔离的进化理解创造和维持物种的过程是现代生物学的一个基本目标。这项拟议的研究将考察行为、生态和后代适合度在限制本土濒危物种科曼奇泉鲤鱼和外来物种羊头小鱼之间杂交的作用。这两个物种在地理上是隔绝的，但最近由于人类活动而发生了身体接触。这项拟议的研究试图确定限制这两个物种个体之间交配的生殖障碍，并影响杂交后代的生存能力和生育能力。繁殖障碍的强度将通过观察科曼奇泉鲤鱼、羊头小鱼及其杂交后代在人工池塘中的交配来确定。科曼奇泉鲤鱼、羊头小鱼和杂交雌性鱼的喜好将通过检测雌性鱼对每种类型雄性鱼同时呈现的视觉和嗅觉线索的反应来测试。还将观察雄性与雄性之间的相互作用，以确定雄性交配行为或竞争能力的物种特定差异。杂交的可行性将通过比较种内和种间交配产生的后代的数量，然后在标准化条件下饲养后代来比较生长率、死亡率和性别比来确定。拟议的研究结果将阐明促进谱系之间差异的机制，并有助于了解北美淡水鱼对杂交的抗性或敏感性。该项目将有助于培训研究生和本科生，并通过海报和展品向公众宣传。学生将接受概念方法以及实验和实地方法的培训。调查结果将在区域和国家会议上以海报和演讲的形式公布，并将在专业期刊和非专业出版物上发表。