Acquisition of Instrumentation for Biophysical Studies: Differential Scanning Calorimeter and Isothermal Titration Calorimeter

购置生物物理研究仪器：差示扫描量热仪和等温滴定量热仪

• 批准号: 0421139
• 负责人: Kathleen Mandell
• 金额: --
• 依托单位: Beloit College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0421139&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Biophysical; Studies; Differential

This award supports the purchase an isothermal titration calorimeter (ITC), a differential scanning calorimeter (DSC), and related software and hardware support for data analysis. The instruments are designed for ease of use, repeated operation (rugged design) and rapid throughput. This new generation of calorimeters provides an order of magnitude improvement in sensitivity and baseline reproducibility with small cell volumes. Thermodynamics of biomolecular systems can be directly measured in a model-independent specific method. DSC and ITC directly measure enthalpy, melting temperature and binding constants for biomolecular systems providing accurate measurements of free energy and entropy of the systems. Specific research projects include: (1) thermodynamics of trinucleotide repeat DNA sequences, (2) determination of the heat capacity of DNA duplex formation, (3) binding constant measurements, and (4) thermodynamics of DNA dumbbell molecular wires. Acquisition of these instruments will provide for biophysical chemical research with undergraduates students, biophysical research training of advanced undergraduate students and development and application of new laboratory modules for undergraduate course work in biochemistry and physical chemistry.

该合同支持购买等温滴定量热计（ITC）、差示扫描量热计（DSC），以及支持数据分析的相关软件和硬件。该仪器的设计便于使用，重复操作（坚固耐用的设计）和快速吞吐量。这种新一代的量热计提供了一个数量级的提高灵敏度和基线再现小细胞体积。生物分子系统的热力学可以用与模型无关的特定方法直接测量。DSC和ITC直接测量生物分子系统的焓、熔化温度和结合常数，提供系统自由能和熵的精确测量。具体研究项目包括：(1)DNA三核苷酸重复序列热力学；(2)DNA双链形成热容的测定；(3)结合常数的测定；(4)DNA哑铃分子线热力学。这些仪器的获得将为本科生的生物物理化学研究提供帮助，为高年级本科生的生物物理研究提供培训，并为生物化学和物理化学的本科课程开发和应用新的实验室模块。